Expression of the plasma membrane Ca2+-ATPase in myogenic cells.

The Journal of Biological Chemistry
A HammesLudwig Neyses

Abstract

To study the physiological function of the plasma membrane calmodulin-dependent calcium ATPase (PMCA) in intact cells, L6 myogenic cell lines stably overexpressing the human PMCA isoform 4CI (= human PMCA isoform 4b) were generated. Several independent L6 clones and controls stably transfected with the empty expression vector were analyzed in detail. The resting cytosolic calcium level in hPMCA4CI-overexpressing muscle cells (measured by the Fura-2 method) was significantly reduced by 20-30% compared with controls. This was shown in a cytosolic window of 1322 single cells (p < 0.01). Furthermore, the differentiation process of these cells was remarkably accelerated compared with control myoblasts and parental nontransfected L6 cells as assessed by multinucleated myotube formation and creatine phosphokinase activity elevation. After 4 and 6 days of differentiation, PMCA-overexpressing L6 cells from four independent clones displayed a 3- and 4-fold higher creatine phosphokinase activity compared with controls (n = 5, p < 0.02). These results may extend the concept of the function of the PMCA from simple prevention of calcium overload to an active involvement in intracellular calcium regulation with potentially important consequen...Continue Reading

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Citations

Oct 3, 2007·Pflügers Archiv : European journal of physiology·Magdalena M SzewczykAshok K Grover
Jun 11, 2005·Journal of Molecular and Cellular Cardiology·Elizabeth J CartwrightLudwig Neyses
Aug 6, 2003·Trends in Molecular Medicine·Oliver Ritter, Ludwig Neyses
Jun 9, 2000·Annual Review of Physiology·H Kiriazis, E G Kranias
Apr 25, 2000·Journal of Molecular and Cellular Cardiology·J AbramowitzJ C Allen
Oct 6, 2009·Nitric Oxide : Biology and Chemistry·Farideh BeigiJoshua M Hare
Apr 21, 2007·Annals of the New York Academy of Sciences·Elizabeth J CartwrightLudwig Neyses
Sep 1, 2004·Biochemical and Biophysical Research Communications·Béla PappChristine Chomienne
Aug 6, 2003·The Journal of Biological Chemistry·Kai SchuhLudwig Neyses
Jun 2, 2017·Physiological Reviews·Nicholas StaffordElizabeth J Cartwright
Mar 28, 2003·American Journal of Physiology. Cell Physiology·Talat AfrozeMansoor Husain
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J PiuholaH Ruskoaho
Jan 12, 2001·Physiological Reviews·E E Strehler, D A Zacharias

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