Expression of the protein tyrosine phosphatase beta2 gene in mouse erythroleukemia cells induces terminal erythroid differentiation.

The Journal of Biological Chemistry
T KumeM Oishi

Abstract

We have cloned cDNA for protein tyrosine phosphatase beta2, which had been implicated in erythroid differentiation of mouse erythroleukemia cells. Expression of cDNA constructs, in which beta2 cDNA is placed under the control of mouse metallothionein-I promoter, by ZnCl2 converted a significant portion (20 to 38%) of the cells to erythroid-like cells, which is 25-50% of the erythroid differentiation efficiency observed by conventional erythroid-inducing agents. Furthermore, introduction and expression of altered protein tyrosine phosphatase beta2 cDNA constructs designed to produce the enzyme lacking the phosphatase activity inhibited erythroid differentiation by 100-20%, depending upon the concentration of erythroid-inducing agents employed. These results strongly suggest that protein tyrosine phosphatase beta2 is involved in triggering erythroid differentiation in mouse erythroleukemia cells.

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Citations

Sep 12, 2000·Journal of Hematotherapy & Stem Cell Research·H AvrahamY Taniguchi
Feb 18, 2003·Molecular and Cellular Biology·Takamune TakahashiThomas O Daniel
Oct 12, 2000·Biochemical and Biophysical Research Communications·T TomemoriT Shirasawa
Oct 3, 1999·Journal of the American Society of Nephrology : JASN·T TakahashiT O Daniel

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