Abstract
We have cloned cDNA for protein tyrosine phosphatase beta2, which had been implicated in erythroid differentiation of mouse erythroleukemia cells. Expression of cDNA constructs, in which beta2 cDNA is placed under the control of mouse metallothionein-I promoter, by ZnCl2 converted a significant portion (20 to 38%) of the cells to erythroid-like cells, which is 25-50% of the erythroid differentiation efficiency observed by conventional erythroid-inducing agents. Furthermore, introduction and expression of altered protein tyrosine phosphatase beta2 cDNA constructs designed to produce the enzyme lacking the phosphatase activity inhibited erythroid differentiation by 100-20%, depending upon the concentration of erythroid-inducing agents employed. These results strongly suggest that protein tyrosine phosphatase beta2 is involved in triggering erythroid differentiation in mouse erythroleukemia cells.
References
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S H OrkinP Leder
Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Oct 1, 1976·Cell·J GusellaD Housman
Apr 1, 1992·Experimental Cell Research·T WatanabeM Oishi
Aug 1, 1992·Molecular Biology of the Cell·A F LauA L Boynton
Nov 15, 1991·Cell·X H YangW Chia
Jun 1, 1990·Biochemical Genetics·P H BoerM W McBurney
Jul 1, 1989·The Journal of Cell Biology·K KondoM Oishi
Aug 1, 1989·Experimental Cell Research·T WatanabeM Oishi
Jan 1, 1988·European Journal of Immunology·H Karasuyama, F Melchers
Apr 1, 1982·Cell·H BeugT Graf
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·K IgarashiM Yamamoto
Jan 30, 1995·FEBS Letters·D ChidaM Oishi
Mar 10, 1995·The Journal of Biological Chemistry·S H Orkin
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·A OstmanN K Tonks
Feb 10, 1994·Nature·K IgarashiM Yamamoto
Jul 1, 1993·Journal of Biochemistry·H MunakataN Hayashi
Oct 10, 1995·Genomics·T WatanabeM Oishi
Citations
Dec 24, 1997·Brain Research. Molecular Brain Research·Y MukouyamaT Watanabe
Sep 12, 2000·Journal of Hematotherapy & Stem Cell Research·H AvrahamY Taniguchi
Feb 18, 2003·Molecular and Cellular Biology·Takamune TakahashiThomas O Daniel
Oct 12, 2000·Biochemical and Biophysical Research Communications·T TomemoriT Shirasawa
May 29, 2003·The Journal of Cell Biology·Maria Grazia LampugnaniElisabetta Dejana
Jul 29, 2000·Leukemia·P P Tang, F F Wang
Jul 4, 2001·FEBS Letters·R IulianoA Fusco
Mar 29, 2000·Oncogene·T MatsuzakiY Ikawa
Oct 12, 2001·The Journal of Biological Chemistry·T FiaschiG Ramponi
Oct 3, 1999·Journal of the American Society of Nephrology : JASN·T TakahashiT O Daniel