Expression of the PTH/PTHrP receptor in chondrogenic cells during the repair of full-thickness defects of articular cartilage

Osteoarthritis and Cartilage
H MizutaY Hiraki

Abstract

We studied the accumulation of parathyroid hormone (PTH)/PTHrP receptor-positive mesenchymal cells using double immunohistochemistry and examined whether this correlated with the subsequent regeneration of 3-mm-diameter full-thickness defects of articular cartilage. Cylindrical full-thickness articular cartilage defects (3 mm) were artificially created in the femoral trochlea of male adolescent Japanese white rabbits (n = 210) with a hand-drill. Recombinant human PTH(1-84) was then administered into the defect cavities with an osmotic pump for either 2 or 4 weeks post-injury. Following PTH treatment, the repair processes in the cartilage defects were histologically examined. Double immunostaining analyses for the PTH/PTH-related peptide (PTHrP) receptor and proliferating cell nuclear antigen (PCNA) in the regenerating tissues were then performed. Activation of PTH/PTHrP receptor signaling by hPTH(1-84) results in the inhibition of chondrogenic differentiation in full-thickness articular cartilage defects. At the conclusion of the 2-week PTH treatment, the defect cavities were filled with undifferentiated mesenchymal cells, which were similar to the controls. In addition, almost all of these cells localized at the center of the ...Continue Reading

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Citations

Oct 19, 2011·Cell and Tissue Research·Hiroaki TsuruokaKazuhisa Takahashi
Feb 14, 2009·Journal of Bone and Mineral Metabolism·Yoshihisa AnrakuYuji Hiraki
Sep 17, 2009·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·Brian T FeeleyJoseph M Lane
Jan 16, 2008·Clinical Orthopaedics and Related Research·Edwin J JansenRoel Kuijer
Apr 17, 2015·International Journal of Rheumatic Diseases·Sunggun Lee
Feb 2, 2019·Journal of Tissue Engineering and Regenerative Medicine·Garima DwivediMichael D Buschmann

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