Expression of the R2R3-MYB transcription factor TaMYB14 from Trifolium arvense activates proanthocyanidin biosynthesis in the legumes Trifolium repens and Medicago sativa.

Plant Physiology
Kerry R HancockSusanne Rasmussen

Abstract

Proanthocyanidins (PAs) are oligomeric flavonoids and one group of end products of the phenylpropanoid pathway. PAs have been reported to be beneficial for human and animal health and are particularly important in pastoral agricultural systems for improved animal production and reduced greenhouse gas emissions. However, the main forage legumes grown in these systems, such as Trifolium repens and Medicago sativa, do not contain any substantial amounts of PAs in leaves. We have identified from the foliar PA-accumulating legume Trifolium arvense an R2R3-MYB transcription factor, TaMYB14, and provide evidence that this transcription factor is involved in the regulation of PA biosynthesis in legumes. TaMYB14 expression is necessary and sufficient to up-regulate late steps of the phenylpropanoid pathway and to induce PA biosynthesis. RNA interference silencing of TaMYB14 resulted in almost complete cessation of PA biosynthesis in T. arvense, whereas Nicotiana tabacum, M. sativa, and T. repens plants constitutively expressing TaMYB14 synthesized and accumulated PAs in leaves up to 1.8% dry matter. Targeted liquid chromatography-multistage tandem mass spectrometry analysis identified foliar PAs up to degree of polymerization 6 in leaf ...Continue Reading

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Citations

Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Véronique CheynierStefan Martens
Mar 26, 2015·Applied Microbiology and Biotechnology·Meiliang ZhouYanmin Wu
Nov 27, 2014·Frontiers in Plant Science·Aidyn Mouradov, German Spangenberg
Feb 1, 2014·Natural Product Reports·Azra GholamiAlain Goossens
Dec 21, 2017·Plants·Bipei Zhang, Andrea Schrader
Dec 27, 2016·Frontiers in Plant Science·Kathy E SchwinnKevin M Davies
Jun 12, 2020·Critical Reviews in Biotechnology·Kai FengAi-Sheng Xiong
May 10, 2018·Frontiers in Plant Science·Nicola M Capstaff, Anthony J Miller
Apr 10, 2021·The Plant Journal : for Cell and Molecular Biology·Wenxing ChenZhengrong Luo
May 9, 2021·The Plant Journal : for Cell and Molecular Biology·Lorenzo BarchiGiovanni Giuliano

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