Expression of the rat adrenomedullin receptor or a putative human adrenomedullin receptor does not correlate with adrenomedullin binding or functional response

Biochemical and Biophysical Research Communications
S P KennedyR J Hill

Abstract

There has been considerable difficulty in defining distinct adrenomedullin (AM) binding sites and function in vivo. However, a rat adrenomedullin receptor (rAMR) and a putative human adrenomedullin receptor (hAMR) have recently been reported. We attempted to confirm and extend the pharmacological characterization of these cloned receptors. COS-7 cells transfected with rAMR or epitope tagged rAMR display abundant rAMR mRNA expression and cell-surface receptor localization. Specific 125I-AM binding is detected in transfected cells; however, similar levels of binding are also detected in cells transfected with vector DNA alone. This AM binding site fails to mediate any changes in cAMP in response to AM. In contrast, Swiss 3T3 cells, expressing specific endogenous AM receptors, display AM binding and functional cAMP responses. Transfection studies performed with the putative hAMR yield similar results. These data suggest that the proposed rAMR and hAMR do not represent authentic adrenomedullin receptors.

References

Oct 27, 1995·The Journal of Biological Chemistry·S KapasA J Clark
Apr 15, 1994·Biochemical and Biophysical Research Communications·Y IshizakaT Eto
Jul 1, 1996·Clinical Science·A M RichardsJ G Lainchbury
Sep 20, 1996·The Journal of Biological Chemistry·M J MillerF Cuttitta
Dec 6, 1996·The Journal of Biological Chemistry·J AiyarK G Chandy
Jan 1, 1997·Current Opinion in Nephrology and Hypertension·K Kitamura, T Eto

❮ Previous
Next ❯

Citations

Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·D L Hay
Jun 14, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hideyuki KobayashiAkihiko Wada
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Béla KisYoichi Ueta
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hideyuki KobayashiAkihiko Wada
Jul 1, 1999·Clinical and Experimental Pharmacology & Physiology·T EtoJ Kato
Sep 23, 2000·Brain Research. Molecular Brain Research·H KobayashiA Wada
Apr 20, 2002·Journal of Neuroendocrinology·B KisY Ueta
Jan 5, 2002·Peptides·D L Hay, D M Smith
Apr 2, 2003·Regulatory Peptides·E ZudaireF Cuttitta
Mar 29, 2002·Microscopy Research and Technique·José López, Natalia Cuesta
Mar 29, 2002·Microscopy Research and Technique·Alfredo Martínez
Aug 17, 2011·Nuclear Medicine and Biology·Yan FuAlain Fournier
Jul 23, 2003·British Journal of Pharmacology·Sophie BuysJean-Michel Senard
Jan 18, 2017·The Journal of Clinical Investigation·Daniel O KecheleKathleen M Caron
Jan 30, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G S RalphD Krige
Mar 29, 2002·Microscopy Research and Technique·Mercedes GarayoaLuis M Montuenga
Mar 29, 2002·Microscopy Research and Technique·Kazuo KitamuraTanenao Eto
Apr 4, 2000·Archives of Andrology·F RossiV Santiemma
Jul 23, 2004·Physiological Reviews·Susan D Brain, Andrew D Grant
Apr 27, 2000·Endocrine Reviews·J P HinsonD M Smith
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Sep 27, 2019·Science Advances·Emily LorenzenThomas P Sakmar
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Leonid L NikitenkoMargaret C P Rees
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T NishikimiK Kangawa
Feb 14, 2002·Journal of Applied Physiology·Anselm T BäumerMichael Böhm
Nov 1, 2011·Toxins·Eric K DumasA Darise Farris
Apr 17, 2021·ACS Pharmacology & Translational Science·Max MeyrathAndy Chevigné
Apr 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Alan Le MercierStefan Offermanns
Jan 5, 2002·Peptides·B KisY Ueta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.