PMID: 9182715Jun 1, 1997Paper

Expression of the surface antigen 4F2hc affects system-L-like neutral-amino-acid-transport activity in mammalian cells

The Biochemical Journal
S BröerB Hamprecht

Abstract

Mammalian cells possess a variety of amino acid-transport systems with overlapping substrate specificity. System L is one of the major amino acid-transport systems of non-epithelial cells. By expression cloning we have recently demonstrated that the surface antigen 4F2hc (CD98) is a necessary component for expression of system-L-like amino acid-transport activity in C6-BU-1 rat glioma cells [Bröer, Bröer and Hamprecht (1995) Biochem. J. 312, 863-870]. 4F2hc mRNA was detected in CHO cells, COS cells, activated lymphocytes isolated from mouse spleen and primary cultures of astrocytes. In all these cell types, Na+-independent isoleucine transport was mediated by system L. No contribution of system y+L to isoleucine or arginine transport was detected in C6-BU-1 cells. In lymphocytes, both system-L-like amino acid-transport activity and 4F2hc mRNA levels increased after treatment with phorbol ester plus ionomycin. Antisense oligonucleotides caused modest inhibition of Na+-independent isoleucine transport in C6-BU-1 cells and primary cultures of astroglial cells, whereas arginine transport was unaffected. Overexpression of 4F2hc cDNA in CHO cells resulted in an increase in Na+-independent isoleucine transport.

Citations

Nov 18, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R DuelliL R Drewes
Sep 8, 2001·American Journal of Physiology. Cell Physiology·C A WagnerS Bröer
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Mar 29, 2000·The Journal of Nutrition·Q R Smith
Mar 19, 2008·Molecular and Cellular Biochemistry·João S AmaralPatrício Soares-da-Silva
Jul 20, 2002·Human Cell·Hiroshi NawashiroYoshikatsu Kanai
May 30, 2017·Frontiers in Immunology·Etienne Masle-FarquharStefan Bröer
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Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R EstévezM Palacín
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