Expression of the transcriptional regulator Egr-1 in experimental glomerulonephritis: requirement for mesangial cell proliferation

Kidney International
H D RupprechtH O Schoecklmann


The early growth response gene-1 (Egr-1), a zinc finger transcriptional regulator, was induced in a rat model of mesangioproliferative glomerulonephritis (GN). Northern blot analysis revealed a maximal 14.9-fold increase in glomerular Egr-1 mRNA at day 6 of GN. By immunohistochemistry Egr-1 protein expression was demonstrated to be mainly confined to glomerular mesangial cells (MC). To test whether Egr-1 directly regulates MC proliferation, cultured MCs were stimulated with platelet-derived growth factor (PDGF) after preincubation with different Egr-1 antisense oligonucleotides (ASOs). PDGF-induced rise in 3H-thymidine uptake by 83% and almost completely abrogated increase in MC number. We conclude that Egr-1 induction is of critical importance for PDGF-induced mitogenic signaling in MCs, and inhibition of Egr-1 in vivo may offer an approach to oppose glomerular MC proliferation in glomerular inflammatory disease.


Dec 31, 2003·The Journal of Surgical Research·Yuko WadaJun Amano
Dec 15, 2000·Kidney International·M PieperH O Schöcklmann
Aug 30, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Danuta Sabuda-WidemannCornelia Blume
Mar 11, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Li-Chun HoYau-Sheng Tsai
Dec 14, 2005·Alcoholism, Clinical and Experimental Research·M T Pritchard, L E Nagy
Jan 31, 2004·Annals of the New York Academy of Sciences·Véronique BaronDan Mercola
Aug 25, 2010·Clinical and Experimental Immunology·J MatheniaX K Zhang
Dec 18, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Susumu KatsumaGozoh Tsujimoto
Oct 30, 2003·American Journal of Physiology. Renal Physiology·Naoko HashimotoHirofumi Makino
Dec 14, 2007·Journal of the American Society of Nephrology : JASN·Jürgen FloegeCharles E Alpers
Feb 18, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tetyana KhomenkoLongchuan Chen
Apr 26, 2014·Journal of the American Society of Nephrology : JASN·Hans-Joachim Anders, Liliana Schaefer
Mar 15, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Amanda J EdgleyWarwick P Anderson
Oct 2, 2015·Inflammation·Wang-Dong XuYi Liu


Sep 1, 1991·Kidney International·Jürgen FloegeW G Couser
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E HallahanR R Weichselbaum
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Christy, D Nathans
Nov 1, 1989·Molecular and Cellular Biology·B Christy, D Nathans
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Lau, D Nathans
Jan 1, 1995·Kidney International·A ShimizuN Yamanaka
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T L BurgessT B Kramer

Related Concepts

Zinc Fingers
Membranoproliferative Glomerulonephritis, Type II
Immediate-Early Proteins
Gene Expression
Cell Division Phases
Egr1 protein, rat
Poly(A) Tail
DNA Helix Destabilizing Proteins

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