PMID: 8960108Oct 1, 1996Paper

Expression of thrombopoietin and thrombopoietin receptor MPL in human leukemia-lymphoma and solid tumor cell lines

Leukemia Research
G GrafH G Drexler

Abstract

Thrombopoietin (TPO) is the major regulator of platelet production in vivo and is the ligand for the MPL receptor. In an effort to determine the distribution of TPO and MPL in the different hematopoietic cell types and in various types of tissue, we examined the mRNA expression of this ligand-receptor pair in two series of human leukemia-lymphoma cell lines and of solid tumor cancer cell lines using northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. At the northern blot mRNA level, 8/15 (53%) megakaryocytic and 3/11 (27%) erythroid leukemia cell lines expressed MPL mRNA; except for one positive monocytic cell line, the remaining 78 pre B-cell, B-cell, plasma cell, T-cell, NK cell, myeloid, monocytic and Hodgkin/anaplastic large cell lymphoma (ALCL)-derived cell lines were negative. No MPL message was detected in any of the 23 solid tumor cell lines established from 21 different tumors. In order to examine whether a low level of MPL expression could be detected, 51 leukemia cell lines were investigated with the RT-PCR technique. By this technique, MPL message was seen in many more cell types: 13/26 (50%) of non-erythromegakaryocytic cell lines and in nearly all megakaryocytic (14/15, 93%) and er...Continue Reading

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·D C FosterA F Ching
Nov 1, 1994·Current Biology : CB·J E Groopman
Sep 2, 1994·Science·A L GurneyM W Moore
Jun 16, 1994·Nature·F WendlingW Vainchenker
Dec 1, 1995·Leukemia & Lymphoma·H T Hassan, H G Drexler

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Citations

Jul 1, 1997·Leukemia Research·C BredouxH G Drexler
Jan 28, 1999·Leukemia Research·W S Alexander, N A Nicola
Jan 28, 1999·Leukemia Research·E Archimbaud, X Thomas
Aug 5, 1998·Cancer Genetics and Cytogenetics·M L Martín RamosM A Fernández-Peralta
May 6, 1999·Bioelectrochemistry and Bioenergetics·J FengX Q Zhang
Sep 9, 2005·International Journal of Hematology·Mark Levis, Donald Small
Nov 4, 2017·Scientific Reports·Amanda E AuEmma C Josefsson

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