Expression of thymic stromal lymphopoietin in equine recurrent airway obstruction

Veterinary Immunology and Immunopathology
J Klukowska-RötzlerJ Janda

Abstract

Thymic stromal lymphopoietin (TSLP) is a cytokine involved in lymphocyte development. In humans and mice, TSLP drives the differentiation of T helper 2 (Th2) cells and the development of allergic inflammation. The equine TSLP gene has been previously identified and characterized, but its role in the pathogenesis of equine allergic diseases is not known. Our objective was to assess the expression of TSLP in bronchoalveolar lavage (BAL) cells and in primary bronchial epithelial cells (BEC) isolated from horses with recurrent airway obstruction (RAO). RNA was isolated from BAL cells sampled from clinical cases of RAO (n=8) and from control horses (n=12). Furthermore, BAL samples were taken from an additional group of 8 RAO-susceptible and 8 control horses when on pasture (remission) and after 30 days of exposure to moldy hay (exacerbation). In order to study epithelial cells as a potential source of TSLP, cultures of primary bronchial epithelial cells (BEC) were established from 6 RAO-affected and 6 healthy horses and stimulated in vitro with hay dust solution (HDS). Expression of TSLP mRNA was assessed by quantitative real-time RT-PCR (qPCR). Clinical RAO-cases had higher TSLP expression in BAL than control horses (p<0.05). In an...Continue Reading

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Apr 27, 2010·Veterinary Immunology and Immunopathology·J Klukowska-RötzlerJ Janda
Oct 12, 2010·American Journal of Respiratory Cell and Molecular Biology·Mathilde LeclereJean-Pierre Lavoie

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Citations

Nov 26, 2013·The Veterinary Clinics of North America. Equine Practice·Valerie A Fadok
Oct 17, 2018·PeerJ·Laurence TessierDorothee Bienzle

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