PMID: 16618975Apr 19, 2006Paper

Expression of thyroid hormone receptor isoforms down-regulated by thyroid hormone in human medulloblastoma cells

Endocrine Journal
Tsuyoshi MondenKikuo Kasai

Abstract

The role of thyroid hormone (T3) in the regulation of growth and development of the central nervous system including the cerebellum has been well established. However, the effects of thyroid hormone on malignant tumors derived from the cerebellum remain poorly understood. Our analysis mainly focused on expression levels of TR isoforms and the effects of thyroid hormone in human medulloblastoma HTB-185 cells. Northern blot analysis revealed TRalpha2 mRNA but not TRalpha1, beta1 or beta2 mRNA in the cell. The TRalpha1 and TRbeta1 mRNAs were detected only by RT-PCR method and TRbeta2 was not expressed. Incubation of T3 for 24 h decreased TRalpha1, TRalpha2 and TRbeta1 mRNA. Addition of actinomycin D caused an acute increase in the basal TR mRNA levels and the rate of decrease of all kinds of TR isoform mRNA was accelerated in the T3-treated groups compared to controls, indicating that the stability of TR mRNA was affected by T3. Incubation with cycloheximide also blocked a decrease in TR mRNA levels in the T3-treated HTB-185 cells suggesting that down-regulation of TR mRNA required the synthesis of new protein. Our data provide novel evidence for the expression of TRs down-regulated by T3 in HTB-185 cells, suggesting that TR expre...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F AnielloJ Nunez
Mar 26, 1990·Molecular and Cellular Endocrinology·C B Cook, R J Koenig
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J BradleyC Weinberger
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M LebelJ Puymirat
Dec 31, 1997·The Journal of Biological Chemistry·T MondenA N Hollenberg
Jan 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D BaasL L Sarliève
Oct 25, 2000·Molecular and Cellular Biology·G R Williams
Mar 20, 2001·Gene·J Guhaniyogi, G Brewer
Feb 18, 2004·The Cerebellum·Noriyuki KoibuchiWilliam W Chin
Oct 23, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Agnes Kenessey, Kaie Ojamaa
Jul 12, 2005·Endocrine Journal·Yoshiyuki KariyaHironobu Sasano

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Citations

Oct 12, 2019·International Journal of Molecular Sciences·Yu-Chin LiuKwang-Huei Lin
Jul 31, 2013·Arquivos brasileiros de endocrinologia e metabologia·Miriane de OliveiraCélia Regina Nogueira
Nov 13, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jordi BlancoDomènec J Sanchez
Dec 4, 2014·Arquivos brasileiros de endocrinologia e metabologia·Miriane de OliveiraCélia Regina Nogueira

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