Expression of thyrotropin-releasing hormone messenger RNA in human pituitary adenomas with follicle-stimulating hormone immunoreactivity.

Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists
C BadiuM Coculescu

Abstract

To assess the correlation between thyrotropin-releasing hormone (TRH) messenger RNA (mRNA) and the immunoreactive type of human pituitary adenomas. Twenty-eight patients (14 to 73 years old) who had pituitary adenomas (18 nonfunctioning adenomas, 8 growth hormone-secreting adenomas, and 2 prolactinomas) underwent surgical treatment. Pituitaries removed at autopsy from four patients without evidence of pituitary disease were used as controls. Fragments of pituitary adenomas were processed for TRH mRNA by in situ hybridization (radioactive and nonradioactive) and for TRH peptide and anterior pituitary hormones (b-thyrotropin, b-follicle-stimulating hormone [FSH], bluteinizing hormone [LH], prolactin, and growth hormone) by immunohistochemistry with use of the avidinbiotin technique. Quantitative immunohistochemical studies were performed by using image analysis software. The signal was considered positive when more than 5% of the cells were stained. Cells expressing TRH mRNA were detected in 22 of 28 pituitary adenomas--15 of 18 nonfunctioning pituitary adenomas, 5 of 8 growth hormone-secreting adenomas, and both prolactinomas. TRH peptide was revealed in only 10 adenomas, all expressing TRH mRNA as well. All but one nonfunctioni...Continue Reading

References

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·E BäzD K Lüdecke
Jun 1, 1991·Pathology, Research and Practice·F PeillonD Joubert
May 1, 1986·The Journal of Clinical Endocrinology and Metabolism·S L AsaH S Smyth
Apr 10, 1980·Nature·T HökfeltM Schultzberg
Jan 21, 1982·The New England Journal of Medicine·I M Jackson
Jun 1, 1995·The Journal of Endocrinology·P G Farnworth
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 1, 1994·Molecular and Cellular Endocrinology·R J Ashworth
Dec 17, 1997·The Journal of Clinical Endocrinology and Metabolism·M YamadaM Mori

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.

Related Papers

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Chang XuLei Ci
© 2022 Meta ULC. All rights reserved