PMID: 8952858Jan 1, 1996Paper

Expression of tissue-type plasminogen activator, plasminogen activator inhibitor and von Willebrand factor in the supernatant of endothelial cell cultures in response to the seeding of adenocarcinoma cell line HRT-18

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
M MorgantiA Sagripanti

Abstract

Plasminogen activator inhibitor (PAI-1), tissue type plasminogen activator (tPA) and von Willebrand factor (vWF) concentrations were measured by ELISA in the supernatant of the following cultures: endothelial cells from human umbilical vein (HUVEC); human colon cancer cells (HRT-18); and co-culture cells of HUVEC + HRT-18. No measurable amount of the three substances was found in the supernatant of HRT-18 cell culture. Compared to the value in the HUVEC supernatant, in the UVEC/HRT-18 co-cultures, tPA concentration was significantly lower (P = 0.0047), PAI-1 significantly higher (P = 0.026) and vWF also significantly higher (P = 0.0048). These data indicate that HRT-18 tumor cells do not produce tPA, PAI-1 and vWF; however, these tumor cells induce endothelial cells to change the production of these substances. As a consequence, the interaction between tumor and endothelial cells in vivo may lead to depression of fibrinolysis and enhancement of platelet adhesion.

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Citations

Dec 2, 2000·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M MorgantiC Mittermayer
Nov 7, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M MorgantiR Giardino
Apr 18, 2012·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·D R WeissR Eckstein
Jun 7, 2000·Journal of the American College of Cardiology·F L Li-Saw-HeeG Y Lip
Feb 13, 2007·Journal of the American College of Cardiology·Kensuke NomaYukihito Higashi

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