Expression of TNF-α system members in bovine ovarian follicles and the effects of TNF-α or dexamethasone on preantral follicle survival, development and ultrastructure in vitro

Animal Reproduction Science
A W B SilvaJ R V Silva

Abstract

This study was conducted to detect the protein expression of TNF-α system members (TNF-α/TNFR1/TNFR2) in bovine ovarian follicles and to evaluate the effects of TNF-α or dexamethasone on the survival and growth of primordial follicles in vitro, as well as on gene expression in cultured ovarian tissue. It was hypothesized that TNF-α induces follicular atresia in ovarian tissues cultured in vitro, and that dexamethasone suppresses the production of endogenous TNF-α, which can improve follicle viability in vitro. Ovarian fragments were cultured for 6days in α-MEM+supplemented with TNF-α (0, 1, 10, 100 or 200ng/ml) or dexamethasone (0, 1, 10, 100 or 200ng/ml). After culture, the expression of mRNAs for BCL-2, BAX, P53, TNF-α, and CASP3 and CASP6 were evaluated. Immunohistochemical results showed that the TNF-α system members, were detected in bovine preantral and antral follicles. After 6days, the TNF-α (10ng/ml) treatment reduced the percentage of normal preantral follicles and increased the number of TUNEL-positive cells in cultured tissue. Dexamethasone (10ng/ml) during 6days of culture did maintain the percentage of normal follicles and the ultrastructure of follicles, while the presence of TNF-α or dexamethasone did not influe...Continue Reading

Citations

May 10, 2020·Zygote : the Biology of Gametes and Early Embryos·José R V SilvaAnderson W B Silva
Aug 28, 2020·Zygote : the Biology of Gametes and Early Embryos·P A A BarrosoJ R V Silva
Jul 25, 2021·International Journal of Molecular Sciences·Alexander G Kuzma-HuntLaura A Favetta
Aug 26, 2021·Zygote : the Biology of Gametes and Early Embryos·Larissa Zamparone BergamoMarcelo Marcondes Seneda
Dec 3, 2021·Zygote : the Biology of Gametes and Early Embryos·Larissa Zamparone BergamoMarcelo Marcondes Seneda

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