Expression of Toll-like receptor -7 and -9 in B cell subsets from patients with primary Sjögren's syndrome

PloS One
Marie KarlsenSilke Appel

Abstract

Sjögren's syndrome (SS) is a rheumatic autoimmune disease characterized by inflammation of exocrine glands. As autoantibodies are present in a majority of patients, B cells have been suggested to play an important role in onset and development of the disease. Toll-like receptors (TLRs) are pattern recognition receptors triggering innate immune responses. Since an increased expression of TLRs has been detected in other rheumatic diseases the purpose of this study was to explore TLRs in B cells of SS patients. The expression of TLR-7 and -9 in B cell subsets of 25 patients with primary SS (pSS) and 25 healthy controls was analysed in peripheral blood using flow cytometry and real time quantitative PCR. We detected similar levels of CD19+ B cells in pSS patients and healthy controls. An increased number of naïve B cells, as well as fewer pre-switched memory B cells were found in pSS patients. No significant differences were observed in TLR-7 and -9 expression in B cells between pSS patients and healthy controls. This study shows that pSS patients have an alteration in the B cell subpopulation composition compared to controls, with less pre-switched memory B cells and more naïve B cells. We did not detect any significant disparitie...Continue Reading

References

Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Mar 27, 2002·International Reviews of Immunology·J C PoeT F Tedder
May 15, 2002·Annals of the Rheumatic Diseases·C VitaliUNKNOWN European Study Group on Classification Criteria for Sjögren's Syndrome
Dec 22, 2004·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M D MignognaA Wolff
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabelle Béatrice Bekeredjian-DingGunther Hartmann
Mar 17, 2006·European Journal of Immunology·Claudia R Ruprecht, Antonio Lanzavecchia
Oct 26, 2006·Nature Reviews. Immunology·Ann Marshak-Rothstein
Apr 17, 2007·Current Opinion in Immunology·Antonio Lanzavecchia, Federica Sallusto
Feb 26, 2008·Autoimmunity Reviews·Almut Meyer-Bahlburg, David J Rawlings
Mar 15, 2008·The Journal of Clinical Investigation·Donna L ThibaultPaul J Utz
Dec 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Lingyan ZhengChi Yang
May 1, 2009·Genes and Immunity·E S EmamianK L Moser
Apr 20, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Lingyan ZhengChi Yang
May 25, 2010·Arthritis and Rheumatism·Pierre YouinouJacques-Olivier Pers
Nov 26, 2010·Nucleic Acids Research·Christina KahramanoglouNicholas M Luscombe
Dec 1, 2010·Journal of Autoimmunity·Kelly J McKelveyPaul A Hessian
Dec 24, 2010·Molecular Systems Biology·Emma LundbergMathias Uhlen
Jan 15, 2011·Scandinavian Journal of Rheumatology·L G GøranssonR Omdal
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jul 23, 2011·Immunology Letters·Roland JonssonSilke Appel
May 9, 2012·Arthritis Care & Research·S C ShiboskiUNKNOWN Sjögren's International Collaborative Clinical Alliance (SICCA) Research Groups
Nov 15, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sun-Hee HwangAnna-Marie Fairhurst
Nov 7, 2013·JAMA : the Journal of the American Medical Association·Roland JonssonGunnel Henriksson
Mar 4, 2014·Journal of Autoimmunity·Thomas GuerrierChristophe Jamin
Apr 15, 2014·Annals of Internal Medicine·Valérie Devauchelle-PensecAlain Saraux

❮ Previous
Next ❯

Citations

Apr 12, 2017·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jeremy KiripolskyJill M Kramer
Aug 15, 2018·International Journal of Molecular Sciences·Hideki NakamuraAtsushi Kawakami
Jun 24, 2017·Journal of Ophthalmology·Hua WangQiang-Xiang Li
Apr 4, 2021·International Journal of Molecular Sciences·Toshimasa ShimizuAtsushi Kawakami

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS

Software Mentioned

FlowJo
GraphPad
Prism

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.