Expression of toll-like receptor 4 and 2 in horse lungs

Veterinary Research
Sarabjeet Singh SuriBaljit Singh

Abstract

Toll-like receptor (TLR) is a key component in launching innate immune response to microbial challenge. TLR4 and TLR2 are recognized as specific receptors for components of Gram-negative and Gram-positive bacteria, respectively. Horses are extremely sensitive to endotoxin-induced cardiopulmonary distress and mortality which causes significant economic losses. To date, there are no data on the expression of TLR4 and TLR2 in horse lungs. Therefore, we examined the expression of TLR4 and TLR2 in lungs from normal or Escherichia coli lipopolysaccharide (E. coli LPS; 50 ng/kg; iv) treated horses. We also studied the impact of the depletion of pulmonary intravascular macrophages (PIM) on TLR4 and TLR2 expression in normal or LPS-treated horses. RT-PCR showed TLR4 mRNA but not TLR2, in normal horse lungs. PIM depletion reduced TLR4 mRNA expression without affecting TLR2. The LPS treatment increased the expression of TLR4 and TLR2 mRNA in normal and PIM-depleted horses compared to normal saline-treated horses. Light and electron microscopic immunocytochemistry showed TLR4 protein in PIM, alveolar macrophages and septal endothelium in lungs from normal or LPS-treated horses. Immuno-gold electron microscopy showed TLR4 in PIM and dual-la...Continue Reading

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Citations

Sep 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaoren TangSalomon Amar
Dec 29, 2015·American Journal of Veterinary Research·Jin-Wen ChenLawrence R Soma
Mar 1, 2017·Veterinary Immunology and Immunopathology·Sandra SchönigerHeinz-Adolf Schoon
Sep 9, 2010·Cell and Tissue Research·David SchnebergerBaljit Singh
Oct 19, 2010·Cell and Tissue Research·Tracey J Coffey, Dirk Werling
Oct 12, 2010·Cell and Tissue Research·Thomas W JungiDirk Werling
Dec 13, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Annerose BerndtN Edward Robinson
Mar 22, 2008·Pediatric Research·Noah H HillmanSuhas G Kallapur
Nov 4, 2010·International Journal of Experimental Pathology·David SchnebergerBaljit Singh
Jan 10, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·David SchnebergerBaljit Singh
May 23, 2012·Equine Veterinary Journal·A H Werners, C E Bryant
Jun 5, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Karin Aharonson-RazBaljit Singh
Jan 19, 2017·Molecular Medicine Reports·Lingjie MaXiaoming Liu
Jun 24, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·David SchnebergerBaljit Singh
Jan 7, 2017·BMC Genomics·Alicja PacholewskaVincent Gerber
Nov 5, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stacy L AndersonBaljit Singh
Jul 3, 2013·Veterinary Immunology and Immunopathology·Ingrid WaldschmidtMari-Capucine Dupuis
Oct 13, 2015·Veterinary Immunology and Immunopathology·Yadu BalachandranBaljit Singh
Dec 22, 2009·Animal Reproduction Science·D M NashE A Lane
Feb 5, 2011·Veterinary Immunology and Immunopathology·Ayshea M QuintanaGisela Soboll Hussey

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