Expression of toll-like receptor 4 and MD-2 gene and protein in Kupffer cells after ischemia-reperfusion in rat liver graft

World Journal of Gastroenterology : WJG
Yong PengShou-Bai Li

Abstract

To investigate the expression of toll-like receptor 4 (TLR4) and MD-2 gene and protein in Kupffer cells (KCs) and their role in ischemia-reperfusion (IR) injury of rat liver graft. KCs were isolated at 0 (control group), 2, 12, 24 h (IR group) following IR in rat liver graft. mRNA expression of TLR4 and MD-2 was detected by RT-PCR analysis, protein expression of TLR4/MD-2 was detected by flow cytometric (FCM) analysis, and tumor necrosis factor-alpha (TNF-alpha) level in supernatant was measured by ELISA. Then isolated KCs were incubated with anti-TLR4 polyclonal antibody (anti-TLR4 group), and TNF-alpha level was measured again. The mRNA and protein expression of TLR4/MD-2 and the level of TNF-alpha in IR group increased significantly at 2 h following IR, and reached the maximum at 12 h, and slightly decreased at 24 h, but were still significantly higher than those in the control group (P<0.01). The expression of these factors was markedly decreased after anti-TLR4 antibody treatment as compared with the IR group (P<0.01). Lipopolysaccharide (LPS) following IR can up-regulate TLR4/MD-2 gene and protein expression in KCs, and synthesize cytokine TNF-alpha. Anti TLR4 antibody can inhibit the production of TNF-alpha induced by LP...Continue Reading

References

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Citations

Mar 17, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P Boros, J S Bromberg
Dec 12, 2007·Journal of Gastroenterology and Hepatology·Toshiyuki KitazawaHiroshi Fukui
Feb 26, 2014·Current Opinion in Organ Transplantation·Tomasz DziodzioJohann Pratschke
Feb 13, 2007·Current Opinion in Nephrology and Hypertension·Christopher Y LuJianlin Chen
Jan 28, 2012·Allergologia et immunopathologia·Bahaa K A Abdel-Salam, Abd-Alla A A Sayed
Jun 23, 2007·Transplant Immunology·Peter BorosJonathan S Bromberg

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