Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis

Experimental and Therapeutic Medicine
Yujing Fan, Bingrong Liu

Abstract

Patients with ulcerative colitis (UC) have a high risk of developing colorectal cancer. The aim of the present study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the colonic mucosa of patients with UC. Colonic mucosal biopsy specimens were collected during colonoscopy from 30 patients with UC and 30 patients with normal findings as controls. The protein and mRNA expression levels of TLRs 1-4 and TLR9 were measured by immunohistochemistry and reverse transcription-quantitative polymerase chain reaction analysis, respectively. The results showed that the mRNA and protein expression of TLR2, TLR4 and TLR9, but not TLR1 and TLR3, was significantly increased in the colonic mucosa of patients with UC compared with that in the normal controls. TLR (TLR2, TLR4 and TLR9) immunoreactivity was found in the cytoplasm of epithelial cells in the mucosa, and occasionally in the endothelium of small vessels of the stromal tissues. In conclusion, TLR2, TLR4 and TLR9 expression may be important in the biological pathogenesis of UC. TLR alterations in the innate response system may contribute to the pathogenesis of UC.

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Citations

Feb 11, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A A KhanS Warnakulasuriya
Oct 28, 2016·Cancer Chemotherapy and Pharmacology·Ronaldo A RibeiroRoberto C P Lima-Júnior
Apr 22, 2020·ELife·Katarzyna BoguckaKrishnaraj Rajalingam
Jan 26, 2021·International Journal of Nanomedicine·Wei-Lian BaoWeixing Shen
Jul 13, 2021·Inflammatory Bowel Diseases·Babajide A OjoMichael J Rosen

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Methods Mentioned

BETA
biopsy
electrophoresis
biopsies

Software Mentioned

SPSS
iCycler iQ Optical

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