Expression of Toll-like receptors on human platelets

Thrombosis Research
Rio ShirakiMitsuhiro Yokoyama

Abstract

Platelets play a crucial role in arterial thrombosis, which is the main cause of acute coronary syndrome. Some mycobacteriums, such as Chlamydia pneumoniae, were associated with progression of atherosclerosis and they are interacted with Toll-like receptors (TLRs), which have been defined as pathogen-associated molecular pattern recognition molecules in innate immunity. In the present study, we examined whether human platelets express TLRs. Human platelets were obtained from healthy volunteers and the mRNA and protein level of TLRs on platelets and Meg-01 cells, megakaryoblastic cell line, were investigated. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that TLR1 and TLR6 mRNA were expressed in platelets and Meg-01 cells. Furthermore, interferon-gamma up-regulated their mRNA levels in dose and time dependent manners after stimuli. Both TLR1 and TLR6 proteins in platelets were detected by Western blotting, and their expression of platelets was more than that of Meg-01 cells. Flow cytometry analysis revealed the expression of TLR1 and TLR6 on the cell surface of Meg-01 cells. Furthermore, immunohistochemical analysis using human coronary thrombi obtained from patients with acute coronary syndrome confirmed...Continue Reading

Citations

Apr 15, 2014·Cardiovascular Research·Sophie Van LinthoutCarsten Tschöpe
Mar 19, 2014·La Presse médicale·Douglas B CinesJohn W Semple
Jun 18, 2010·Canadian Journal of Physiology and Pharmacology·Archibald McNicol, Sara J Israels
Aug 6, 2011·Seminars in Immunopathology·Adriana Vieira-de-AbreuGuy A Zimmerman
Feb 10, 2006·Blood·Satoshi FujimiPeter H Lapchak
Sep 12, 2008·Journal of Leukocyte Biology·Kun QianJianming Wu
Feb 3, 2009·Immunologic Research·Neil BlumbergRichard P Phipps
Jun 17, 2009·Journal of Cardiovascular Pharmacology·Kazunori HashimotoVirginia M Miller
Dec 17, 2009·Cellular and Molecular Life Sciences : CMLS·Michael R Yeaman
Feb 5, 2010·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Apr 13, 2011·Purinergic Signalling·Martyn P Mahaut-SmithRichard J Evans
Feb 15, 2013·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Meagan O'BrienJeffrey S Berger
Jan 22, 2010·Cellular and Molecular Life Sciences : CMLS·Steven W Kerrigan, Dermot Cox
Dec 18, 2009·Cellular and Molecular Life Sciences : CMLS·John W Semple, John Freedman
Mar 10, 2010·Future Microbiology·Michael R Yeaman
Dec 3, 2014·Journal of Dermatological Science·Risa Tamagawa-Mineoka
Jun 12, 2012·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·O GarraudF Cognasse
Nov 13, 2010·Thrombosis Research·Olivier GarraudFabrice Cognasse
Mar 10, 2016·Thrombosis Research·Caroline M LarkinMarek W Radomski
Oct 10, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·F CognasseO Garraud
May 15, 2007·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·F CognasseO Garraud
Sep 12, 2014·Thrombosis and Haemostasis·Daniel DuerschmiedIngo Ahrens
Jun 27, 2014·Thrombosis and Haemostasis·Sacha F de StoppelaarTom van der Poll
Jul 6, 2014·Thrombosis and Haemostasis·Cornelia SpethCornelia Lass-Flörl
Oct 16, 2010·Journal of Thrombosis and Haemostasis : JTH·C KeaneS W Kerrigan
Sep 17, 2014·Journal of Thrombosis and Haemostasis : JTH·J M HerterA Zarbock
Mar 13, 2015·Experimental Hematology & Oncology·Antoine DewitteJean Ripoche
Jul 3, 2013·International Immunopharmacology·Clive Page, Simon Pitchford
Oct 13, 2005·Experimental Hematology·Fabrice Cognasse, Olivier Garraud
Oct 13, 2015·The American Journal of Cardiology·Kadri Murat GursesKudret Aytemir
Dec 17, 2015·Journal of Thrombosis and Haemostasis : JTH·R D MoriartyD Cox

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