Expression of Toll-like receptors (TLR) and responsiveness to TLR agonists by polarized mouse uterine epithelial cells in culture

Biology of Reproduction
Gisela SobollCharles R Wira

Abstract

The objective of the present study was to examine the expression of Toll-like receptors (TLRs) by mouse uterine epithelial cells and to determine if stimulation of the expressed TLR induces changes in cytokine and/or chemokine secretion. Using RT-PCR, the expression of TLRs 1-6 by mouse uterine epithelial cells was demonstrated, with TLRs 7-9 expressed only periodically. In the absence of pathogen-associated molecular patterns, polarized uterine epithelial cells constitutively secrete interleukin (IL) 1A, cysteine-cysteine ligand (CCL) 2, IL6, granulocyte-macrophage colony-stimulating factor 2 (CSF2), tumor necrosis factor A (TNFA), CSF3, and IL8 in vitro, with levels of cytokines/chemokines secreted into the apical compartment being significantly greater than those released into the basolateral compartment. When added to the apical surface for 48 h before analysis, the TLR2-agonist Pam3Cys-Ser-(Lys)4 and TLR1/6-agonist peptidoglycan increased epithelial cell apical secretion of IL1A, CCL2, and IL6 and apical/basolateral bidirectional secretion of CSF2, TNFA, CSF3, and IL8 when compared to controls. The TLR3-agonist poly (I:C) significantly increased bidirectional secretion of CCL2, IL6, TNFA, and CSF2 and basolateral secretion...Continue Reading

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Citations

May 30, 2008·Journal of Assisted Reproduction and Genetics·Zhilin LiuJoanne S Richards
May 30, 2009·Reproductive Biology and Endocrinology : RB&E·Shan HerathI Martin Sheldon
Apr 30, 2010·Reproductive Biology and Endocrinology : RB&E·Jason W RossRodney D Geisert
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May 12, 2007·Journal of Reproductive Immunology·Reza Aflatoonian, Alireza Fazeli
May 6, 2006·American Journal of Reproductive Immunology : AJRI·Gisela SobollCharles R Wira
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May 9, 2014·American Journal of Reproductive Immunology : AJRI·Severina N Haddad, Charles R Wira
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Feb 5, 2011·Veterinary Immunology and Immunopathology·Ayshea M QuintanaGisela Soboll Hussey
Aug 9, 2016·Journal of Reproductive Immunology·Iain Martin SheldonMatthew Lloyd Turner
Sep 21, 2010·Experimental Dermatology·Margarete NiebuhrThomas Werfel
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Jul 15, 2015·Biology of Reproduction·John E SchjenkenSarah A Robertson
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Mar 6, 2015·Biology of Reproduction·Laura L HealyI Martin Sheldon
Aug 7, 2018·Veterinary Immunology and Immunopathology·Glenn HamonicHeather L Wilson

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