PMID: 9178265May 1, 1997Paper

Expression of Toxoplasma gondii genes in the closely-related apicomplexan parasite Neospora caninum

Molecular and Biochemical Parasitology
D K HoweL D Sibley

Abstract

We have established the feasibility of using Neospora caninum as a heterologous system for the expression of genes from the closely-related parasite Toxoplasma gondii. Plasmid construct containing the lacZ gene from Escherichia coli driven by T. gondii promoters were electroporated into N. caninum parasites, and expression of beta-galactosidase (beta-Gal) activity was assayed enzymatically. In transient assays, expression of beta-Gal driven by the GRA1 promoter was approximately 10-fold higher than the expression obtained with the SAG1 promoter. Enzyme activity was not detected when N. caninum parasites were transfected with a promoterless lacZ construct. Transfection of N. caninum with complete genomic clones of SAG1 or GRA2 from T. gondii yielded parasites that transiently expressed the respective gene products, as detected by immunofluorescence and Western blot. Additionally, these transiently expressed T. gondii proteins appeared by immunofluorescence localization and Triton X-114 partitioning to be correctly targeted in both extracellular and intracellular N. caninum parasites. Heterologous gene expression should be useful for studying the function of specific gene products and may facilitate the identification of genes re...Continue Reading

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Citations

Mar 9, 2004·International Journal for Parasitology·Kami Kim, Louis M Weiss
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fabien BrossierSinisa Urban
Aug 24, 1999·Veterinary Parasitology·J P Dubey
Nov 27, 1999·International Journal for Parasitology·A HemphillA Hehl
May 12, 2011·FEBS Letters·Julien Limenitakis, Dominique Soldati-Favre
Jun 23, 2010·Experimental Parasitology·Qiang LvXichen Zhang
Mar 2, 2010·Trends in Parasitology·José A Fernández-Robledo, Gerardo R Vasta
Aug 7, 2014·Veterinary Parasitology·Caroline M MotaTiago W P Mineo
Jul 18, 2006·Molecular and Biochemical Parasitology·Rajshekhar Y GajiDaniel K Howe
Sep 19, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Stephen J GoodswenJohn T Ellis

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