Expression of transgenes from newcastle disease virus with a segmented genome

Journal of Virology
Qinshan GaoPeter Palese

Abstract

Paramyxoviruses belong to the Paramyxoviridae family of the order Mononegavirales. They have a nonsegmented negative-stranded RNA genome and can cause a number of diseases in humans and animals. We generated a recombinant Newcastle disease virus (NDV) possessing a two-segmented genome. Each genomic segment is flanked by authentic NDV 3' and 5' noncoding termini allowing for efficient replication and transcription. A reporter gene encoding green fluorescent protein (GFP) was inserted into one segment, and a red fluorescent protein dsRed gene was inserted into the other segment in order to easily detect the replication and transcription of segments in infected cells. The rescued viruses grew well and were stable in embryonated chicken eggs over multiple passages. We were able to detect the expression of both reporter genes in the same cell infected with the virus possessing a segmented genome, and viral particles can contain either one or two types of RNA segments. We also rescued a two-segmented virus expressing GFP and the severe acute respiratory syndrome-associated coronavirus spike S protein, which is about 200 kDa. The chimeric virus extends the coding capacity of NDV by 30%, suggesting that the two-segmented NDV can be use...Continue Reading

References

May 1, 1992·The Journal of Surgical Research·K W ReichardJ A Greager
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M EnamiP Palese
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P WhelanG T Wertz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Jun 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A KatoY Nagai
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G NeumannY Kawaoka
Oct 9, 1999·Journal of Virology·E FodorA García-Sastre
Dec 2, 1999·The Journal of General Virology·A Römer-OberdörferT C Mettenleiter
Nov 2, 2001·Journal of Virology·T NakayaP Palese
May 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andrew L PecoraRobert M Lorence
May 15, 2002·The EMBO Journal·Monika RagerRoberto Cattaneo
Apr 10, 2004·Journal of Neuro-oncology·L K CsataryC M Csatary
Dec 18, 2004·Cancer Gene Therapy·Huijie BianVolker Schirrmacher
Jan 18, 2006·Journal of Virology·Luis Martinez-SobridoJoan E Durbin
Apr 14, 2006·Journal of Virology·Makoto TakedaYusuke Yanagi
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Man-Seong ParkPeter Palese
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jutta VeitsAngela Römer-Oberdörfer
Sep 29, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sabine WagnerLaszlo K Csatary
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Mar 10, 2007·Current Cancer Drug Targets·Robert M LorenceMichael K Bamat
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Joshua M DiNapoliAlexander Bukreyev
Aug 10, 2007·Journal of Virology·Bernadette Crescenzo-Chaigne, Sylvie van der Werf
Sep 7, 2007·Cancer Research·Adam VigilAdolfo García-Sastre

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Citations

Nov 11, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Joyce WongYuman Fong
Feb 18, 2010·Future Virology·Ronald M IorioPaul J Mahon
Oct 8, 2015·Veterinary Microbiology·S ParidaA C Banyard
Jul 1, 2016·Annual Review of Phytopathology·Andrew O Jackson, Zhenghe Li
Jan 1, 2013·Uirusu·Hideki Kondo
May 27, 2017·Annual Review of Virology·Anice C Lowen
Dec 28, 2016·Veterinary Microbiology·Kiril M DimitrovPatti J Miller
Feb 13, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tonya L TaylorClaudio L Afonso

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