PMID: 2119809Aug 27, 1990Paper

Expression of translation initiation factor 4A from yeast and mouse in Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
A PratPatrick Linder

Abstract

The eukaryotic translation initiation factor 4A (eIF-4A) plays an important role in regulating initiation. To analyze its function in yeast, we carried out a mutational analysis of the TIF1 and TIF2 genes, which encode eIF-4A. Expression of these two yeast genes has also been investigated at the transcriptional level and it has been found that both are expressed in wild-type yeast cells. Analysis of the expression of eIF-4A-beta-galactosidase fusion proteins reveals that the TIF2 gene is more highly expressed than the TIF1 gene. Interestingly, the yeast eIF-4A protein shows a high degree of amino acid sequence similarity to the mouse homologue. However, we find that the mammalian factor does not support protein synthesis in yeast either in vivo or in vitro.

References

Mar 1, 1990·Molecular and Cellular Biology·F RozenN Sonenberg
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T H ChangJ Abelson
Jan 12, 1989·Nature·P LinderP P Slonimski
Jul 11, 1989·Nucleic Acids Research·J C DorsmanL A Grivell
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S BlumH Trachsel
Aug 1, 1989·Current Genetics·P P MüllerP Linder
Apr 1, 1988·Molecular and Cellular Biology·S B Baim, F Sherman
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D C FryA S Mildvan
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Jul 8, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bernard Kwabi-AddoMichael Ittmann
May 11, 2011·Chemistry : a European Journal·Sandra SchinzelMartin Kaupp
Jul 10, 2013·The Journal of Experimental Medicine·André Ballesteros-TatoTroy D Randall

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Citations

Dec 1, 1993·Current Genetics·A StotzP Linder
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick SchützUlrich Baumann
Aug 10, 2010·The Journal of Biological Chemistry·Michael Altmann, Patrick Linder
Aug 1, 1992·Antonie van Leeuwenhoek·P Linder
Apr 5, 2005·Mechanisms of Development·Greco HernándezRolando Rivera-Pomar
Sep 22, 2012·RNA Biology·Alexandra Z Andreou, Dagmar Klostermeier
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Nov 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Linder, A Prat
Jun 16, 2001·The Journal of Biological Chemistry·W LiC G Proud
Sep 29, 1995·The Journal of Biological Chemistry·C L WeiJ W Hershey

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