Expression of tuberculosis antigen ESAT-6 in Nicotiana tabacum using a potato virus X-based vector

Tuberculosis
Alicia ZeladaAngel Cataldi

Abstract

A good candidate antigen to create a therapeutic vaccine against TB is the ESAT-6 protein. Antigens produced in plants have already been successfully used as experimental vaccines, and small single-stranded RNA plant viruses have emerged as promising tools to rapidly express large amounts of foreign proteins in susceptible host plants. Here, we present the expression of ESAT-6 protein in Nicotiana tabacum using a vector based on potato virus X (PVX). The complete ESAT-6 open reading frame is expressed as a fusion protein with the 2A peptide of Foot and Mouth Disease Virus and the amino terminal of the PVX coat protein (CP) (PVXESAT-6). This strategy allows the production of free CP and ESAT-6 as well as fused ESAT-2A-CP to obtain recombinant chimaeric virions expressing ESAT-6 at the surface to be used as particulate antigen in vaccination. ESAT-6 expression was tested in agroinfiltrated tobacco leaves and products of the expected molecular masses corresponding to cleaved CP and ESAT-2A-CP fusion protein were observed, with ESAT-6 yields ranging from 0.5% to 1% of total soluble protein. Our study describes for the first time the expression of the ESAT-6 protein in tobacco plants using a PVX-derived vector. This strategy should ...Continue Reading

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Citations

Apr 21, 2011·Applied Biochemistry and Biotechnology·Hongqi FuXiaokun Li
Aug 3, 2010·Expert Review of Vaccines·Rurick K SalyaevNatalya I Rekoslavskaya
Apr 10, 2009·Biotechnology Advances·Siddharth TiwariRakesh Tuli
Dec 5, 2015·Frontiers in Plant Science·Chiara LicoSelene Baschieri
Dec 22, 2006·Tuberculosis·Yuri L DorokhovJoseph G Atabekov
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Jul 1, 2008·Experimental Parasitology·Gisela FerraroMarina Clemente
Aug 11, 2018·Reviews in Medical Virology·Garry A Luke, Martin D Ryan
Jul 10, 2018·Briefings in Functional Genomics·Kashyap Kumar DubeyPratyoosh Shukla
Jan 4, 2020·Biotechnology and Applied Biochemistry·Kiran SabaMohammad Tahir Waheed
Feb 17, 2015·Expert Review of Vaccines·Sergio Rosales-MendozaCarlos Angulo
Mar 7, 2019·Frontiers in Plant Science·Juliane RöderUlrich Commandeur
May 19, 2017·Frontiers in Microbiology·Tsung-Hsien ChenYau-Heiu Hsu
Jul 14, 2017·Frontiers in Plant Science·Juliane RöderUlrich Commandeur
Jan 1, 2008·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·J J JoensuuJ E Brandle
Aug 3, 2010·Expert Review of Vaccines·Tatiana V KomarovaYuri L Dorokhov

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