Expression of tumor necrosis factor-alpha in IgM+ B-cells from bovine leukemia virus-infected lymphocytotic sheep

Veterinary Immunology and Immunopathology
Tatsufumi UsuiMisao Onuma

Abstract

Tumor necrosis factor (TNF)-alpha is thought to be one of the cytokines that account for bovine leukemia virus (BLV)-induced B-cell lymphoproliferative disorder, however, information on TNF-alpha expression in B-cells is limited. In this study, the expression of TNF-alpha in IgM(+) B-cells from BLV-infected sheep with or without lymphocytosis was determined. Freshly isolated IgM(+) B-cells from three sheep with lymphocytosis constitutively transcribed TNF-alpha mRNA. Although TNF-alpha mRNA expression in IgM(+) B-cells was transiently up-regulated after cell culture, TNF-alpha mRNA expression was markedly higher in lymphocytotic sheep when compared to that of non-lymphocytotic sheep or uninfected sheep. Expression of membrane-bound TNF-alpha on IgM(+) B-cells was also augmented in lymphocytotic sheep. TNF-alpha expression in lymphocytotic sheep may support the proliferation of B-cells.

References

Mar 4, 1998·European Journal of Immunology·M GrellP Scheurich
Jun 24, 1999·The Journal of Veterinary Medical Science·H KabeyaM Onuma
Aug 16, 2001·The Journal of Veterinary Medical Science·H KabeyaM Onuma
Mar 19, 2002·Cellular Signalling·David J MacEwan
Jun 28, 2003·Veterinary Microbiology·Satoru KonnaiMisao Onuma

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Citations

Jun 5, 2020·Voprosy virusologii·I Y EzdakovaT V Stepanova

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