Expression of tumor protein D52-like genes in childhood leukemia at diagnosis: clinical and sample considerations

Leukemia Research
Draga BarbaricJennifer A Byrne

Abstract

The tumor protein D52 gene or protein is frequently overexpressed in several carcinomas, and has been identified as a B cell differentiation marker. D52-like genes are also differentially expressed in particular haematological malignancies, where transcript or protein levels may reflect cellular proliferative or differentiative status. We used RT-PCR to analyse the expression of three D52-like genes in bone marrow at the time of ALL or AML diagnosis in children. Whereas D53 transcripts were undetectable in all samples, D52 and D54 transcripts were frequently detected in ALL and AML, where they were frequently co-expressed. While D52 and D54 transcripts were detected in T-ALL and pre-B ALL at comparable frequencies, D52 was less frequently detected in ALL bone marrow with hyperdiploid karyotypes, compared with samples with normal karyotypes. We also found that total RNA yields significantly differed according to D52 and D54 expression status, and that bone marrow freezer storage time (up to 945 days) differed significantly according to D52 expression status. These results indicate that D52-like genes are not ubiquitously expressed in leukemic bone marrow in children, and that RNA sample parameters may influence measures of gene ...Continue Reading

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Citations

Feb 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Apostolos ZaravinosDemetrios A Spandidos
May 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jennifer A ByrneRobert K Bright
Jun 4, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Michael W GranerEmmanuel Katsanis
May 12, 2015·Nature Genetics·Kajsa PaulssonBertil Johansson
Aug 27, 2013·Cell Cycle·Yuyan ChenJennifer A Byrne
Mar 10, 2015·Cancer Biotherapy & Radiopharmaceuticals·Jiapeng XuQingping Cai
Jan 30, 2015·Cancer Biotherapy & Radiopharmaceuticals·Mei YangShan Wu
Sep 12, 2018·Journal of Cellular Biochemistry·Mihyang HaYun Hak Kim
May 19, 2021·Computers in Biology and Medicine·Raissa Coelho AndradeFernando Regla Vargas

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