Expression of two alternative sigma factors of Synechococcus sp. strain PCC 7002 is modulated by carbon and nitrogen stress.

Microbiology
Laurie F CaslakeD A Bryant

Abstract

The sigB and sigC genes, encoding two alternative sigma factors of the unicellular marine cyanobacterium Synechococcus sp. PCC 7002, were cloned and characterized. Strains in which the sigB and sigC genes were insertionally inactivated were viable under standard laboratory conditions, indicating that SigB and SigC are group 2 sigma factors. Starvation for either nitrogen or carbon caused an increase in sigB mRNA levels. Transcripts for the sigC gene initially increased but then decreased during nitrogen and carbon starvation. The SigC protein could not be identified in cyanobacterial extracts using antisera to Synechococcus sp. PCC 7002 SigA or RpoD from Bacillus subtilis. The ratio of the principal vegetative sigma factor, SigA, to SigB decreased during either nitrogen starvation or carbon starvation, and the levels of SigB also increased in the sigC mutant strain. These results imply that SigB and SigC play roles in modifying transcription in response to changes in carbon and nitrogen availability in this cyanobacterium.

References

Jul 1, 1992·Bioscience, Biotechnology, and Biochemistry·K TanakaH Takahashi
Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Mar 1, 1989·Journal of Bacteriology·J L WealandR Hirschberg
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C BinnieR Losick
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
Mar 11, 1987·Nucleic Acids Research·C B Harley, R P Reynolds
Jan 4, 1969·Nature·R R BurgessE K Bautz
Jan 1, 1983·Molecular & General Genetics : MGG·N Tomioka, M Sugiura
Sep 1, 1995·Microbiological Reviews·W H Mager, A J De Kruijff
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Aug 1, 1993·Molecular Microbiology·B Bukau
Jun 1, 1994·Current Opinion in Cell Biology·J A Goodrich, R Tjian
Dec 1, 1993·Genes & Development·A J DombroskiC A Gross
Jan 1, 1993·Annual Review of Microbiology·R KolterA Tormo
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A J DombroskiC A Gross
Apr 10, 1997·Nature·M Ptashne, A Gann

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Citations

May 28, 2010·Journal of Molecular Evolution·Sarah M Chiang, Herb E Schellhorn
Jun 10, 2005·Nucleic Acids Research·Sylvain LemeilleJohannes Geiselmann
Apr 26, 2005·BMC Microbiology·Sylvain LemeilleAmel Latifi
Jun 19, 2007·The Journal of General and Applied Microbiology·Kaori Inoue-SakamotoDonald A Bryant
May 20, 2015·ACS Synthetic Biology·C Josh RameyNanette R Boyle
Oct 7, 2003·Annual Review of Microbiology·Tanja M Gruber, Carol A Gross
Apr 29, 2016·Journal of Applied Microbiology·Y FuW Zhang
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Aaron Kaplan, Leonora Reinhold
Nov 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nathan G WalworthEric A Webb
Oct 4, 2006·Molecular Systems Biology·Andrew C TolonenSallie W Chisholm
Mar 12, 2008·Physiologia Plantarum·Takashi OsanaiKan Tanaka
Oct 20, 2009·Gene Regulation and Systems Biology·Sousuke Imamura, Munehiko Asayama
Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
Nov 25, 2020·Trends in Microbiology·Amit SrivastavaPratyoosh Shukla
Jan 17, 2020·Biotechnology Advances·Amit SrivastavaRoman Sobotka

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