Expression of type X collagen and matrix calcification in three-dimensional cultures of immortalized temperature-sensitive chondrocytes derived from adult human articular cartilage

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
B O OyajobiB M Stringer

Abstract

Chondrocytes isolated from normal adult human articular cartilage were infected with a retroviral vector encoding a temperature-sensitive mutant of the simian virus 40 large tumor antigen and a linked geneticin (G418)-resistance marker. G418-resistant colonies were then isolated, ring-cloned, and expanded in serum-containing media. Several immortalized chondrocyte cell lines were established from the clones that survived, some of which have been maintained in continuous culture for over 2 years. Despite serial subcultures and maintenance as monolayers, these cells retain expression of markers specific for cells of the lineage, namely type II collagen and aggrecan, detected immunocytochemically. We also examined the phenotype of three of these immortalized cell lines (designated HAC [human articular chondrocyte]) using a pellet culture system, and in this report, we present evidence that a prototype of these lines (HAC-F cells) expresses markers normally associated with hypertrophic chondrocytes. When HAC-F cells were cultivated in centrifuge tubes, for periods of up to 63 days, at 39 degrees C with mild and intermittent centrifugation they continued to express both lineage markers; total type II collagen/pellet remained stable,...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·E Fanning, R Knippers
Apr 1, 1992·Analytical Biochemistry·M E AdamsL J Sandell
Jan 1, 1991·Experimental Cell Research·M PacificiI M Shapiro
Oct 1, 1988·Experimental Cell Research·W E HortonJ R Hassell
Apr 1, 1994·The Journal of Clinical Investigation·A P HollanderA R Poole
Jan 1, 1994·Critical Reviews in Oncogenesis·T M Bryan, R R Reddel
Jan 1, 1995·International Review of Cytology·R CanceddaP Castagnola
Mar 1, 1995·In Vitro Cellular & Developmental Biology. Animal·B BenoitM Adolphe
Jul 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M AliniA R Poole
Oct 1, 1994·Annals of the Rheumatic Diseases·C W Archer
Dec 1, 1994·The Journal of Clinical Investigation·M B GoldringJ F Apperley
Jan 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B O OyajobiR G Russell
Aug 1, 1993·Calcified Tissue International·Y NakagawaT Yamamuro
Dec 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H Muir
Jun 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P V BodineB S Komm
Mar 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J S PriceL J Sandell
Sep 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·I GirkontaiteK Von der Mark
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N MatagaM Noda
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A L BoskeyI Binderman
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C ShukunamiY Hiraki
Sep 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M D'Angelo, M Pacifici
Aug 1, 1995·Clinical Molecular Pathology·D J ButtleA P Hollander

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Citations

Jan 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takeharu NakamataJunya Toguchida
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y Sun, R Kandel
Mar 13, 2003·Biotechnology and Bioengineering·Ronald D GraffGreta M Lee
Apr 27, 2004·Journal of Cellular Biochemistry·Michael J ZuscikRandy N Rosier
Aug 20, 2005·Journal of Biomedical Materials Research. Part a·Kiyoshi YonenoKazuo Tanne
Nov 10, 2005·Journal of Biomedical Materials Research. Part a·Wei-Hung ChenCharng-Bin Yang
Jun 1, 2005·Osteoarthritis and Cartilage·Carsten LübkeManrico Paulitschke

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