PMID: 8587011Jan 1, 1995Paper

Expression of urokinase-type plasminogen activator and plasminogen activator inhibitor in colon disease

Journal of Clinical Laboratory Analysis
S MiseljicJ L Wittliff

Abstract

Surveillance colonoscopy and biopsy are inaccurate methods of predicting the likelihood of ulcerative colitis patients to develop colon carcinoma. We examined uPA and PAI-1 as potential markers for assessing these patients and those with familial polyposis who are at risk of developing colon cancer. For comparison, biopsies of normal colon and Crohn's disease were evaluated. We examined 77 colonic mucosa specimens taken from patients undergoing elective resection for benign and malignant colonic disease. uPA and PAI-1 were measured using a monoclonal antibody-based ELISA kit (American Diagnostica, Greenwich, CT) and expressed as ng/mg extract protein. Intra- and interassay controls of uPA gave CV = 3-4% and CV = 8-9%, respectively, while those for PAI-1 were 6-7% and 10-11%, respectively. The Mann-Whitney test showed that both uPA and PAI-1 expression were significantly higher in colon cancer, chronic ulcerative colitis, and Crohn's disease than in normal colon. uPA in familial polyposis samples was similar to that of normal colon, while PAI-1 was much lower than in normal colon. Neither patient age nor sex appeared to influence the expression of these potential markers in any tissue. The pattern of uPA and PAI-1 expression in ...Continue Reading

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Citations

Oct 12, 2010·International Journal of Colorectal Disease·Petra KrauseIngo Leister
Jan 30, 2008·European Journal of Clinical Investigation·I D PousaJ P Gisbert
Feb 6, 2004·Journal of Surgical Oncology·Nigel P S CrawfordSusan Galandiuk
Oct 11, 2008·The American Journal of Gastroenterology·Ole D LaerumBoye S Nielsen
Jan 21, 2009·Scandinavian Journal of Gastroenterology·Anna SolbergMarie-Louise Ivarsson
Aug 20, 2021·Expert Opinion on Therapeutic Targets·Esmat AbdiMohammad Hassan Esmailnejad

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