PMID: 11905403Mar 22, 2002Paper

Expression of uterine and cervical epithelial cadherin during relaxin-induced growth in pigs

Reproduction : the Official Journal of the Society for the Study of Fertility
Peter L RyanCarol A Bagnell

Abstract

Epithelial cadherin (E-cadherin), a member of the cadherin family of calcium-dependent adhesion molecules, is present in reproductive tissues. Relaxin, a hormone important for uterine and cervical growth in pigs, increases the expression of E-cadherin in the MCF-7 mammary epithelial cell line. The objective of this study was to characterize the expression of E-cadherin during relaxin-induced growth of the uterus and cervix in an immature pig model, independent of high circulating steroids. After administration of relaxin to prepubertal gilts, the uterus and cervix were collected. E-cadherin mRNA and protein were measured by northern and western blot analysis, respectively. A 120 kDa protein band, corresponding to E-cadherin, was detected in all tissues examined. Relaxin significantly (P < 0.05) increased the amount of E-cadherin protein in the uterus (P < 0.05), whereas no significant changes were observed in E-cadherin protein in the cervix. A 4.2 kb E-cadherin transcript was detected in all tissues and E-cadherin mRNA was significantly higher (P < 0.05) in uteri from relaxin-treated gilts compared with control gilts. E-cadherin was localized by immunocytochemistry to the epithelial cells of the uterine and cervical lumen, and...Continue Reading

Citations

Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Eric S Hayes
Jun 16, 2005·Annals of the New York Academy of Sciences·Carol A BagnellFrank F Bartol
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·F F BartolC A Bagnell
Jul 24, 2012·European Journal of Pharmacology·Aline Rosa NascimentoMaria Fatima Magalhaes Lazari

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