Expression of v-cath gene from HearNPV in tobacco confers an antifeedant effect against Helicoverpa armigera

Journal of Biotechnology
Yong ZhangShiyun Chen

Abstract

Biotechnology solutions for insect control on crops largely depend on the expression of Bacillus thuringiensis insecticidal proteins to kill pests. V-CATH, a cathepsin L-like cysteine protease from baculoviruses, has been shown to play an essential role in host insect liquefaction. In this study, the v-cath gene from Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) was cloned into the pBI121 binary vector under the control of CaMV35S promoter, and was expressed in tobacco via Agrobacterium-mediated transformation. PCR and RT-PCR analyses of T(1) kanamycin-resistant tobacco progeny plants confirmed the integration and transcription of the v-cath gene. Using a leaf-disk bioassay, antifeedant activity toward H. armigera was tested. Our result showed that, when feeding the first-instar larvae of H. armigera with leaves of transgenic plants, the v-cath transgene expression has a profound antifeedant effect. Most importantly, the growth and development of the insect were inhibited when transferred from leaf-feeding to artificial diet. Our result demonstrated that v-cath gene from baculovirus induced antifeedant effect against H. armigera, resulted in larval stunting and retarded insect development, and has the potential to ...Continue Reading

References

Aug 1, 1997·The Plant Journal : for Cell and Molecular Biology·P E UrwinH J Atkinson
Oct 1, 1998·The Plant Journal : for Cell and Molecular Biology·K V RaoJ A Gatehouse
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yves CarrièreBruce E Tabashnik
Mar 12, 2004·Annual Review of Entomology·F Moscardi
Feb 14, 2006·Transgenic Research·N FerryA M R Gatehouse
May 13, 2006·Trends in Plant Science·Paul ChristouAngharad M R Gatehouse
Oct 13, 2006·The Journal of General Virology·Juliana Velasco de Castro OliveiraPaolo Marinho de Andrade Zanotto
Nov 3, 2007·Science·Mario SoberónAlejandra Bravo
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts

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