PMID: 15367889Sep 16, 2004Paper

Expression of vacuole membrane protein 1 (VMP1) in spontaneous chronic pancreatitis in the WBN/Kob rat

Pancreas
Pei-Hong JiangNorio Sawabu

Abstract

VMP1 is a stress-induced gene that is overexpressed in acute pancreatitis. Its overexpression promotes the formation of intracellular vacuoles and cell death. We investigated the expression of VMP1 mRNA and its relation to apoptosis in spontaneous chronic pancreatitis in the WBN/Kob rat. Four-week-old male WBN/Kob rats were fed a special breeding diet, MB-3, for 20 weeks. Rats were killed every 4 weeks, and the pancreas was examined. VMP1 mRNA expression was determined by reverse transcriptase polymerase chain reaction with a semiquantitative analysis, direct sequencing, and in situ hybridization. Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) were used to detect cell proliferation and apoptosis, respectively. Vacuolar formation was most prominent at 12 weeks, when chronic pancreatitis occurred. VMP1 mRNA was also strongly expressed at 12 weeks. In situ hybridization revealed VMP1 mRNA was expressed in acinar cells. Apoptosis was increased at 12 and 20 weeks, and PCNA expression was strongest at 16 weeks in the course of chronic pancreatitis. VMP1 mRNA expression paralleled the formation of vacuoles and apoptosis in acin...Continue Reading

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Citations

Mar 9, 2005·Digestive Diseases·Vuk SavkovićHans Bödeker
Dec 7, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qiao YingXianghuo He
Mar 15, 2006·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·R Maynard Case
Mar 29, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alan Ka-Lun KaiIrene Oi-Lin Ng
Jul 15, 2016·Protein Science : a Publication of the Protein Society·Yang MeiSangita C Sinha
Oct 18, 2007·The Journal of Biological Chemistry·Alejandro RopoloMaria I Vaccaro

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