Expression of vanilloid receptor 1 in cultured fibroblast

Experimental Dermatology
Seong-Jin KimYoung-Ho Won

Abstract

Although the vanilloid receptor 1 (VR1) was originally discovered on primary sensory neurons, its broad tissue expression in non-neuronal cells has been reported on. Recently, VR1 expression was clearly demonstrated in a variety of cutaneous components, such as keratinocytes, glandular epithelium, mast cells and sebocytes, except for melanocytes and fibroblasts. However, we demonstrated the VR1 expression in the cultured human skin fibroblasts as follows. Previously cloned human VR1 primers that corresponded to the expected size of 680 bp by reverse transcriptase polymerase chain reaction were identified on the fibroblasts, the same as was noted for the positive control, the HaCaT cells. A positive immunoreactivity of the VR1 was observed both on fibroblasts and on HaCaT cells by Western blotting analysis. Fibroblasts treated with capsaicin, an agonist to the VR1, induced significant changes of the membrane current and the intracellular calcium level, and these changes were antagonized by capsazepin. Capsaicin treatment also showed a positive immunocytochemistry result. Our results suggest the existence of VR1 on fibroblasts; this receptor is likely to be influenced by ligand-dependent activation.

References

Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·E MezeyA Szallasi
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·S W HwangU Oh
Aug 2, 2001·Biochemical and Biophysical Research Communications·M DendaK Matsunaga
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L A BirderM J Caterina
Feb 7, 2002·Biochemical and Biophysical Research Communications·Kaori InoueMitsuhiro Denda
Jul 4, 2002·The Journal of Biological Chemistry·Zoltan OlahMichael J Iadarola
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Michael D SouthallJeffrey B Travers
Feb 6, 2003·The Journal of Biological Chemistry·Constance J ChuJ Michael Walker
Jul 13, 2004·The Journal of Investigative Dermatology·Eniko BodóTamás Bíró

❮ Previous
Next ❯

Citations

Jan 22, 2009·Cancer Research·Ann M BodeZigang Dong
Aug 28, 2007·The Bulletin of Tokyo Dental College·Takashi Suzuki
Jan 26, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·M SulzbergerE Grönniger
Aug 7, 2009·Journal of Dermatological Science·Tae-Young ChoiTae-Jin Yoon
Jul 16, 2010·Experimental Dermatology·Thomas KueperGabriele Vielhaber
Jan 12, 2012·British Journal of Pharmacology·E S FernandesJ E Keeble
Oct 4, 2011·Pain·Anil JainGitta Neufang
Jul 26, 2018·Journal of Veterinary Pharmacology and Therapeutics·Raffaella BarberoGiovanni Re
Jun 17, 2019·The Journal of Rheumatology·Dimitar EvdokimovNurcan Üçeyler
Aug 7, 2019·Experimental and Therapeutic Medicine·Mihaela Adriana IlieDaniel Boda
Jul 3, 2021·International Journal of Molecular Sciences·Michelle D Bagood, R Rivkah Isseroff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.