Expression of vasopressin receptors in ACTH-independent macronodular bilateral adrenal hyperplasia causing Cushing's syndrome: molecular, immunohistochemical and pharmacological correlates

The Journal of Endocrinology
E LouisetH Lefebvre

Abstract

Cortisol secretion in ACTH-independent macronodular adrenal hyperplasia (AIMAH) causing Cushing's syndrome can be controlled by illegitimate receptors. The aim of the present study was to characterize the molecular, immunohistochemical, and pharmacological profiles of vasopressin receptors in cells derived from three patients with AIMAH (H1-H3), in order to evaluate the role of ectopic vasopressin receptors in the physiopathology of hypercortisolism. Expression of mRNAs encoding the vasopressin receptor types (V(1a), V(1b), and V(2)) were analyzed by RT-PCR in adrenal tissues. The presence of V(1a) and V(2) receptors was studied by immunohistochemistry on adrenal sections. The pharmacological profiles of vasopressin receptors involved in the control of cortisol secretion were investigated using the V(1a) receptor antagonist SR49059 and the V(2) receptor agonist [deamino-Cys(1), Val(4), D-Arg(8)]-vasopressin on cultured cells. The V(1a) receptor protein was present and functional in H1 and H3 tissues, whereas the V(1b) receptor was not expressed in any of the tissues. RT-PCR experiments revealed that V(2) receptor mRNAs were detected in the three tissues. In contrast, immunohistochemical and cell incubation studies showed that t...Continue Reading

Citations

Mar 14, 2014·The New England Journal of Medicine·Estelle Louiset, Hervé Lefebvre
Jan 10, 2009·European Journal of Endocrinology·O ZwermannM Reincke
Jun 9, 2009·Best Practice & Research. Clinical Endocrinology & Metabolism·André Lacroix
May 15, 2015·European Journal of Endocrinology·Nada El GhorayebAndré Lacroix
Jun 25, 2015·Frontiers in Endocrinology·Ludivine DrougatBruno Ragazzon
Jul 29, 2018·Der Pathologe·W Saeger
Jul 26, 2017·BMJ Case Reports·Kush Dev Singh JarialAnil Bhansali
Apr 14, 2012·Current Opinion in Endocrinology, Diabetes, and Obesity·David E Schteingart
Jul 19, 2017·Endocrine Pathology·Omalkhaire M AlshaikhShereen Ezzat
Feb 16, 2021·Endocrine·Lucas BouysJérôme Bertherat
Mar 14, 2014·The New England Journal of Medicine·Tetsuo NishikawaHironobu Sasano

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