Expression of vip1/vip2 genes in Escherichia coli and Bacillus thuringiensis and the analysis of their signal peptides

Journal of Applied Microbiology
Y ShiY Pang

Abstract

To determine the expression time courses and high expression level of Vip2A(c) and Vip1A(c) in Bacillus thuringiensis, and survey their insecticidal toxicity and insecticidal spectrum. A kind of new vegetative insecticidal toxin genes encoded by a single operon from B. thuringiensis had been cloned and sequenced. The individual genes, 5-terminus truncated genes and the operon were respectively expressed in Escherichia coli. Only N-terminus deleted Vip2A(c) and Vip1A(c) proteins could be purified by Ni-NTA agarose, while others were processed and their N-terminal signal peptides were cleaved. The individual genes and the operon were also expressed in B. thuringiensis. Both proteins were mostly secreted into the cell supernatants. The expression level of Vip1A(c) was influenced because of the interruption of vip2A(c) gene on the operon. Bioassays showed that neither separate protein nor both performed any toxicity against tested lepidopteran and coleopteran insects. Vip2A(c) and Vip1A(c) have similar secretion mechanism in E. coli and B. thuringiensis. Vip1A(c) remained its high expression level only when being expressed with vip2A(c) gene as an operon in B. thuringiensis. Expression of vip2A(c) and vip1A(c) genes in E. coli and ...Continue Reading

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Citations

Sep 3, 2013·Indian Journal of Microbiology·Hussein H AbulreeshAbdulrahman S A Assaeedi
Aug 2, 2007·Canadian Journal of Microbiology·G T Vilas-BôasO M N Arantes
Jul 1, 2014·Journal of Insect Physiology·Silvia CacciaJuan Ferré
Dec 17, 2014·Toxins·Leopoldo PalmaPrimitivo Caballero
Feb 19, 2013·Journal of Invertebrate Pathology·Patricia Hernández-MartínezJuan Ferré
Apr 3, 2012·Journal of Invertebrate Pathology·Maissa ChakrounJuan Ferré
Jun 7, 2016·International Journal of Biological Macromolecules·Sameh SellamiKaïs Jamoussi
Mar 5, 2016·Microbiology and Molecular Biology Reviews : MMBR·Maissa ChakrounJuan Ferré
Jul 3, 2020·Toxins·Mikel Domínguez-ArrizabalagaPrimitivo Caballero

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