Expression of Vitis amurensis VaERF20 in Arabidopsis thaliana Improves Resistance to Botrytis cinerea and Pseudomonas syringae pv. Tomato DC3000

International Journal of Molecular Sciences
Mengnan WangXiping Wang

Abstract

Ethylene response factor (ERF) transcription factors play important roles in regulating immune responses in plants. In our study, we characterized a member of the ERF transcription factor family, VaERF20, from the Chinese wild Vitis genotype, V. amurensis Rupr "Shuangyou". Phylogenetic analysis indicated that VaERF20 belongs to group IXc of the ERF family, in which many members are known to contribute to fighting pathogen infection. Consistent with this, expression of VaERF20 was induced by treatment with the necrotrophic fungal pathogen Botrytis cinerea (B. cinerea) in "Shuangyou" and V. vinifera "Red Globe". Arabidopsis thaliana plants over-expressing VaERF20 displayed enhanced resistance to B. cinerea and the bacterium Pseudomonas syringae pv. tomato (Pst) DC3000. Patterns of pathogen-induced reactive oxygen species (ROS) accumulation were entirely distinct in B. cinerea and PstDC3000 inoculated plants. Examples of both salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) responsive defense genes were up-regulated after B. cinerea and PstDC3000 inoculation of the VaERF20-overexpressing transgenic A. thaliana plants. Evidence of pattern-triggered immunity (PTI), callose accumulation and stomatal defense, together with incre...Continue Reading

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Citations

Aug 22, 2018·International Journal of Molecular Sciences·Isabel Diaz
Nov 17, 2020·Frontiers in Plant Science·Jiaotong YangTao Zhou
Mar 27, 2021·Frontiers in Plant Science·Zhenyuan ZangWeiguang Yang
Sep 8, 2018·Journal of Agricultural and Food Chemistry·Wenqing YuLin Shen

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Datasets Mentioned

BETA
AY680701

Methods Mentioned

BETA
RNA-Seq
transgenic
PCR

Software Mentioned

SPSS Statistics
Excel
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MEGA

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