PMID: 3757030Sep 12, 1986Paper

Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport

Cell
M J GethingJ Sambrook

Abstract

The hemagglutinin of influenza virus is synthesized as a monomeric subunit that is cotranslationally translocated across the membrane of the rough endoplasmic reticulum. We show that folding and assembly of hemagglutinin monomers into trimeric structures takes approximately 7-10 min and is completed before the protein leaves the endoplasmic reticulum. Mutants of hemagglutinin that fail to be transported from the endoplasmic reticulum are blocked at different stages of the folding pathway. Unfolded molecules of hemagglutinin are associated with a cellular protein of 77 kd that has been shown previously to bind to IgG heavy chain in the endoplasmic reticulum of certain myelomas. We discuss why assembly of native structures is required for transport of proteins through the exocytotic pathway.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
Feb 1, 1986·Journal of Virology·R W DomsA Helenius
Jan 1, 1986·The American Journal of Physiology·J R Subjeck, T T Shyy
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Jan 1, 1986·The Journal of Cell Biology·M C Zuniga, L E Hood
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark
Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Nov 24, 1983·Nature·I G Haas, M Wabl
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik

❮ Previous
Next ❯

Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P S Reddy, R B Corley
Jan 1, 1997·Protein Science : a Publication of the Protein Society·J B ThodenI Rayment
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H R GaskinsE H Leiter
Apr 1, 1990·Journal of Bioenergetics and Biomembranes·D Hoekstra
Jan 1, 1988·Archives of Virology·T A SatoA Sugiura
May 1, 1990·The Journal of Membrane Biology·K Geering
Jan 1, 1987·Immunologic Research·D H MarguliesJ McCluskey
Jan 1, 1991·Immunologic Research·D C DeNagel, S K Pierce
Nov 13, 2008·Cytotechnology·Saeed U Khan, Martin Schröder
Jan 1, 1991·Progress in Lipid Research·J BorénS O Olofsson
Dec 6, 1989·Biochimica Et Biophysica Acta·M F Schmidt
Feb 28, 1990·Biochimica Et Biophysica Acta·J F Hare
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Grossman
May 1, 1991·Toxicology Letters·D T KochevarJ Cooper
Jan 1, 1994·Biotechnology Advances·D D Mosser, B Massie
Aug 1, 1991·Current Opinion in Cell Biology·M J Gething
Jun 1, 1997·Virus Research·E MalvoisinF Wild
Oct 20, 2005·Journal of Bioscience and Bioengineering·T KasuyaK Kitamoto
Apr 7, 2000·Breast Cancer Research and Treatment·P M FernandezS R Patierno
May 1, 1994·Nature Structural Biology·J F SinclairT O Baldwin
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A GonzalezT Gottlieb
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T R Hirst, J Holmgren
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Pillai, D Baltimore
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J RobbM P Neeper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.