Abstract
We identified ZAKI-4 (also designated as DSCR1L1) as a thyroid hormone responsive gene in cultured human skin fibroblasts. Recently it has been reported that ZAKI-4 belongs to an evolutionary conserved family of proteins that function as calcineurin inhibitor. In human, ZAKI-4 and calcineurin are highly expressed in brain, where thyroid hormones play essential roles in the development during fetal and neonatal periods. In the present study, we examined the temporal and spatial expression patterns of ZAKI-4 messenger RNA (mRNA) in control and hypothyroid rat brains. Northern blot analysis revealed that ZAKI-4 mRNA was detected in both cerebral cortex and cerebellum as early as embryonic day (E)18. In the cerebral cortex, the expression level gradually increased with age, reaching a plateau at postnatal day (P)7 and remained constant thereafter until P30. A similar pattern of increase with age was also observed in hypothyroid rats; however, the magnitude of the increase was significantly reduced. In control rats, the fold increase in ZAKI-4 mRNA level from E18 to P17 was 10.8; whereas in hypothyroid rats, it was 7.4. In cerebellum the expression level did not change with age or by thyroid status. In situ hybridization revealed th...Continue Reading
References
May 1, 1992·Journal of Neurochemistry·T KunoC Tanaka
Feb 1, 1991·Molecular Endocrinology·A MuñozJ Bernal
Jan 15, 1991·Biochemical and Biophysical Research Communications·T TakaishiC Tanaka
May 1, 1990·Molecular Endocrinology·Y MurataN Matsui
Oct 1, 1985·Cell·R J MilnerJ G Sutcliffe
Nov 5, 1986·Brain Research·S GotoE Miyamoto
Jan 1, 1987·Annual Review of Physiology·J H Dussault, J Ruel
Aug 9, 1974·Brain Research·J M LauderH Krebs
Oct 1, 1995·European Journal of Endocrinology·J Bernal, J Nunez
Nov 1, 1995·The Journal of Cell Biology·F Shibasaki, F McKeon
Jun 1, 1995·European Journal of Biochemistry·Q SuB Ryffel
Mar 1, 1996·Endocrinology·M A IniguezJ Bernal
Jun 14, 1996·The Journal of Biological Chemistry·T MiyazakiH Seo
Oct 1, 1996·Surgical Neurology·C V BorlonganP R Sanberg
Mar 7, 1998·Cell·D G WinderE R Kandel
Mar 7, 1998·Cell·I M MansuyM E Bach
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·A SíkT F Freund
May 6, 1998·Cell·J D MolkentinE N Olson
Jun 5, 1998·The Journal of Biological Chemistry·C B KleeX Wang
Mar 25, 1999·Cell·G R Crabtree
Apr 7, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·J Krebs
Apr 9, 1999·Science·H G WangJ C Reed
May 25, 1999·Cell Transplantation·C V BorlonganS Watanabe
Nov 24, 1999·The Journal of Biological Chemistry·A AsaiT Kirino
Mar 18, 2000·The Journal of Biological Chemistry·B RothermelR S Williams
Jun 22, 2000·Human Molecular Genetics·J J FuentesS de la Luna
Citations
Aug 31, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Babak TahvildariDavid A McCormick
Jun 26, 2007·Cell and Tissue Research·Nuno da CostaKin-Chow Chang
Sep 5, 2007·The European Journal of Neuroscience·Sílvia PortaMaria L Arbonés
May 18, 2012·Endocrinology·J H Duncan BassettGraham R Williams