Expression of ZmGA20ox cDNA alters plant morphology and increases biomass production of switchgrass (Panicum virgatum L.)

Plant Biotechnology Journal
Phat T DoZhanyuan J Zhang

Abstract

Switchgrass (Panicum virgatum L.) is considered a model herbaceous energy crop for the USA, for its adaptation to marginal land, low rainfall and nutrient-deficient soils; however, its low biomass yield is one of several constraints, and this might be rectified by modulating plant growth regulator levels. In this study, we have determined whether the expression of the Zea mays gibberellin 20-oxidase (ZmGA20ox) cDNA in switchgrass will improve biomass production. The ZmGA20ox gene was placed under the control of constitutive CaMV35S promoter with a strong TMV omega enhancer, and introduced into switchgrass via Agrobacterium-mediated transformation. The transgene integration and expression levels of ZmGA20ox in T0 plants were analysed using Southern blot and qRT-PCR. Under glasshouse conditions, selected transgenic plants exhibited longer leaves, internodes and tillers, which resulted in twofold increased biomass. These phenotypic alterations correlated with the levels of transgene expression and the particular gibberellin content. Expression of ZmGA20ox also affected the expression of genes coding for key enzymes in lignin biosynthesis. Our results suggest that the employment of ectopic ZmGA20ox and selection for natural variant...Continue Reading

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Citations

Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Yuhan DongShenchun Qu
Sep 28, 2017·BioMed Research International·Julián Mario Peña-CastroLorena Amaya-Delgado
Mar 7, 2020·Frontiers in Plant Science·Huibin WangLingfei Xu
Feb 11, 2021·Plant Biotechnology Journal·Yanrong LiuWanjun Zhang

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Methods Mentioned

BETA
transgenic
PCR
phosphotransferase
PCRs

Software Mentioned

GenScript
SPSS
Image Analysis
MetaMorph Microscopy Automation

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