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Expression optimization and synthetic gene networks in cell-free systems

Nucleic Acids Research

Apr 1, 2012

David K KarigMitchel J Doktycz

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Abstract

Synthetic biology offers great promise to a variety of applications through the forward engineering of biological function. Most efforts in this field have focused on employing living cells, yet cell-free approaches offer simpler and more flexible contexts. Here, we evaluate cell-free r...read more

Mentioned in this Paper

Feedback, Biochemical
Early Promoters, Genetic
Repressor Molecules
Cell-Free System
Tetracycline resistance-encoding transposon repressor protein
Promoter
Lac Repressors
Synthetic Genes
Escherichia coli
Entamoeba coli
4
172
Paper Details
References
  • References83
  • Citations33
  • References83
  • Citations33
1234

Expression optimization and synthetic gene networks in cell-free systems

Nucleic Acids Research

Apr 1, 2012

David K KarigMitchel J Doktycz

PMID: 22180537

DOI: 10.1093/nar/gkr1191

Abstract

Synthetic biology offers great promise to a variety of applications through the forward engineering of biological function. Most efforts in this field have focused on employing living cells, yet cell-free approaches offer simpler and more flexible contexts. Here, we evaluate cell-free r...read more

Mentioned in this Paper

Feedback, Biochemical
Early Promoters, Genetic
Repressor Molecules
Cell-Free System
Tetracycline resistance-encoding transposon repressor protein
4
172

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Paper Details
References
  • References83
  • Citations33
  • References83
  • Citations33
1234

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