Expression pattern and signalling pathways in neutrophil like HL-60 cells after treatment with estrogen receptor selective ligands

Molecular and Cellular Endocrinology
Chellakkan Selvanesan Blesson, Lena Sahlin

Abstract

Estrogens play a role in the regulation of genes associated with inflammation and immunity in neutrophils. Estrogen signalling is mediated by estrogen receptor (ER)α, ERβ, and G-protein-coupled estrogen receptor-1 (GPER). The mechanisms by which estrogen regulate genes in neutrophils are poorly understood. Our aim was to identify the presence of ERs and to characterize estrogen responsive genes in terminally differentiated neutrophil like HL-60 (nHL-60) cells using estradiol and selective ER agonists. ERs were identified by Western blotting and immunocytochemistry. Microarray technique was used to screen for differentially expressed genes and the selected genes were verified by quantitative PCR. We show the presence of functional ERα, ERβ and GPER. Microarray analysis showed the presence of genes that are uniquely regulated by a single ligand and also genes that are regulated by multiple ligands. We conclude that ERs are functionally active in nHL-60 cells regulating genes involved in key physiological functions.

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Citations

Apr 12, 2016·Developmental and Comparative Immunology·Ewa SzwejserMagdalena Chadzinska
Feb 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiali YinHaojun Luo
Sep 26, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabel CabasAlfonsa García-Ayala
Sep 26, 2020·Frontiers in Endocrinology·Christian David Hernández-SilvaAna Laura Pereira-Suárez
Jan 13, 2021·International Journal of Molecular Sciences·Jaqueline S da SilvaGisele Zapata-Sudo
Aug 8, 2021·Cells·Karolina NowakWioletta Ratajczak-Wrona

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