Expression pattern of inflammatory response genes and their regulatory micrornas in bovine oviductal cells in response to lipopolysaccharide: implication for early embryonic development

PloS One
Sally IbrahimDawit Tesfaye

Abstract

In the present study, we used an in vitro model to investigate the response of the oviduct with respect to inflammatory mediators and their regulatory microRNAs in case of bacterial infection and subsequent association with embryo survival. For this, we conducted two experiments. In the first experiment, cultured primary bovine oviductal cells (BOEC) were challenged with lipopolysaccharide (LPS) for 24h and the temporal expression pattern of inflammatory mediators and their regulatory microRNAs were measured at 0, 3, 6, 12, 24 and 48h after LPS treatment. Intriguingly, the temporal patterns of all miRNAs except miR-21 were significantly up-regulated at 6h after LPS treatment. Whereas, we observed significant overexpression of pro-inflammatory mediators as tumor necrosis factor alpha (TNFα) and interleukin-1 beta (IL1β) after LPS challenge for 24h. On the other hand, the expression level of essential elements like oviductal glycoprotein 1 (OVGP1) and insulin-like growth factor 2 (IGF2) was significantly decreased in challenged groups compared with control. Moreover, miR-155, miR-146a, miR-223, miR-21, miR-16 and miR-215 have shown a clear suppression in challenged group after LPS treatment. In the 2nd experiment there were four ...Continue Reading

References

May 1, 1975·The Journal of Infectious Diseases·H S Kantor
Oct 16, 1999·Reviews of Reproduction·K Hardy
May 11, 2000·Obstetrical & Gynecological Survey·J A McGregor, J I French
Jan 5, 2002·Physiological Reviews·Wulf Dröge
Feb 28, 2002·Trends in Immunology·Sarah G HarrisRichard P Phipps
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·I M SheldonH Dobson
Mar 26, 2003·Reproduction in Domestic Animals = Zuchthygiene·R H F Hunter
Sep 30, 2004·Nature Immunology·Donald N CookDavid A Schwartz
Dec 14, 2005·Current Opinion in Immunology·Luke A J O'Neill
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan CostineanCarlo M Croce
Sep 30, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Regine RottmayerStefan Hiendleder
May 9, 2007·Infectious Diseases in Obstetrics and Gynecology·Yogesh Kumar JaiswalKaushik Deb
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Esmerina TiliCarlo Maria Croce
Oct 30, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Shan HerathI Martin Sheldon
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Raj Raghupathy, Jaroslaw Kalinka
May 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Andrew W HorneAnne E King
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·M G Diskin, D G Morris
Sep 16, 2008·Journal of Animal Science·M Z Carletti, L K Christenson
Oct 28, 2008·Seminars in Reproductive Medicine·Qun Pan, Nasser Chegini
May 30, 2009·Reproductive Biology and Endocrinology : RB&E·Shan HerathI Martin Sheldon
Dec 4, 2009·Carcinogenesis·Aaron J SchetterCurtis C Harris
Mar 31, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy J MurphyPatricia A Pioli
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Frank WeinbergNavdeep S Chandel
Jul 21, 2010·Nature Immunology·Mahima SwamyAdrian Hayday
Aug 17, 2010·BMC Genomics·Caroline G WalkerMurray D Mitchell
Jan 21, 2011·Theriogenology·Mateus José SudanoFernanda da Cruz Landim-Alvarenga
Jan 25, 2011·Animal Reproduction Science·S W WalshA C O Evans

❮ Previous
Next ❯

Citations

Sep 26, 2017·Reproduction, Fertility, and Development·Katheryn L CernyPhillip J Bridges
Dec 20, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Barbara P S ComettiMarinella Rosselli
Nov 2, 2018·Asian-Australasian Journal of Animal Sciences·Shanjiang ZhaoHuabin Zhu
Nov 23, 2019·Journal of Cellular and Molecular Medicine·Kangfeng JiangGanzhen Deng
Jun 27, 2019·American Journal of Reproductive Immunology : AJRI·Yuki NakamuraKoumei Shirasuna
Feb 25, 2020·Journal of Dairy Science·F M DalaneziH Langoni
May 1, 2018·Zygote : the Biology of Gametes and Early Embryos·Rasoul KowsarAkio Miyamoto
Dec 10, 2020·Theriogenology·Fariborz MoghadamMohammad Hossein Nasr Esfahani
Mar 9, 2021·Frontiers in Veterinary Science·Manoel Neres Santos JuniorLucas Miranda Marques
Mar 23, 2021·Frontiers in Veterinary Science·Rosane MazzarellaJuliano Coelho da Silveira

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
Assay

Software Mentioned

Primer3
Quantity One

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis