Expression patterns of Anaplasma marginale Msp2 variants change in response to growth in cattle, and tick cells versus mammalian cells.

PloS One
Adela S Oliva ChávezUlrike G Munderloh

Abstract

Antigenic variation of major surface proteins is considered an immune-evasive maneuver used by pathogens as divergent as bacteria and protozoa. Likewise, major surface protein 2 (Msp2) of the tick-borne pathogen, Anaplasma marginale, is thought to be involved in antigenic variation to evade the mammalian host immune response. However, this dynamic process also works in the tick vector in the absence of immune selection pressure. We examined Msp2 variants expressed during infection of four tick and two mammalian cell-lines to determine if the presence of certain variants correlated with specific host cell types. Anaplasma marginale colonies differed in their development and appearance in each of the cell lines (P<0.001). Using Western blots probed with two Msp2-monospecific and one Msp2-monoclonal antibodies, we detected expression of variants with differences in molecular weight. Immunofluorescence-assay revealed that specific antibodies bound from 25 to 60% of colonies, depending on the host cell-line (P<0.001). Molecular analysis of cloned variant-encoding genes demonstrated expression of different predominant variants in tick (V1) and mammalian (V2) cell-lines. Analysis of the putative secondary structure of the variants rev...Continue Reading

References

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Citations

Apr 22, 2015·Experimental & Applied Acarology·Jonathan D OliverUlrike G Munderloh
Mar 18, 2015·PloS One·Geoffrey E LynnUlrike G Munderloh

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Datasets Mentioned

BETA
JN703154
AY132312
EU499670

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Psipred
ProtParam
ClustalW
MacVector
MacVector®
SigmaPlot
MEGA

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