Expression patterns of novel genes during mouse preimplantation embryogenesis

Molecular Reproduction and Development
G L TemelesR M Schultz

Abstract

Little is known about the repertoire of genes expressed following zygotic gene activation, which occurs during the two-cell stage in the mouse. As an initial attempt to isolate novel genes, we used previously prepared two-cell and two-cell subtraction cDNA libraries (Rothstein et al., Genes Dev 6:1190-1201, 1992) to isolate a panel of seven cDNA clones. Three cDNAs had no match in the current DNA sequence data banks and three others revealed sequence homology to portions of sequences in the data banks. One cDNA was 90% homologous to the ras-related gene Krev/rap 1A. The temporal patterns of expression of these genes during oocyte maturation and preimplantation development were analyzed by a reverse transcription-polymerase chain reaction (RT-PCR) assay developed to measure relative levels of mRNAs. Three distinct temporal patterns of expression, designated Classes 1-3, were found. The two Class 1 genes displayed an actin-like pattern, with a gradual decline in expression during oocyte maturation and through the two-cell stage, followed by increases at the eight-cell and/or blastocyst stages. The four genes in Class 2 were expressed at relatively high levels during oocyte maturation and through the one-cell stage and then declin...Continue Reading

References

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Citations

Jan 1, 1996·Molecular Reproduction and Development·C M CorrickI Kola
Jun 1, 1996·Molecular Reproduction and Development·D R Brison, R M Schultz
Jul 1, 1996·Molecular Reproduction and Development·C J WilliamsG S Kopf
Oct 10, 1997·Molecular Reproduction and Development·J V FlemingW D Rees
Sep 13, 2001·Molecular Reproduction and Development·J E AndersonR S Prather
Jun 1, 1995·Molecular Reproduction and Development·G O Babalola, R M Schultz
Mar 26, 2003·Developmental Biology·Paula SteinRichard M Schultz
Dec 10, 2002·Reproductive Biomedicine Online·Martin H JohnsonMargot L Day
Aug 1, 1995·Zygote : the Biology of Gametes and Early Embryos·F RevelV Duranthon
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·J W Zimmermann, R M Schultz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K DasS K Dey
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·B C PariaS K Dey
Oct 1, 1998·Nucleic Acids Research·W Davis, R M Schultz
Oct 23, 2002·Biology of Reproduction·Claude RobertMarc-André Sirard
Jan 21, 2003·Biology of Reproduction·Daisuke KigamiHiroshi Imai
Nov 25, 2003·Biology of Reproduction·Bolette BjerregaardPoul Maddox-Hyttel
Oct 31, 2003·Biology of Reproduction·Bolette BjerregaardPoul Maddox-Hyttel
Jul 13, 1999·The Laryngoscope·A B RobinsonT R Sanford
May 22, 2004·European Journal of Biochemistry·Bhavesh JoshiRosemary Jagus
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·R E LeachD R Armant
Apr 21, 2007·BMC Developmental Biology·Diana LuciferoJacquetta M Trasler
Aug 22, 2007·PLoS Genetics·Kimberly J ReeseMarisa S Bartolomei

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