Abstract
To determine the association of the KL-6 epitope (sugar moiety) with MUC1 and its distribution on the ocular surface of human non-dry and dry eyes. The human ocular surface was examined immunohistochemically and immunoelectron microscopically using monoclonal antibody (mAb) KL-6, which recognize a carbohydrate epitope of MUC1. The expression patterns of KL-6 epitope in corneal and conjunctival cells were examined by impression cytology from 24 non-dry eye volunteers and 43 dry-eye patients. In the cornea, bulbar conjunctiva, and limbus epithelium, mAb KL-6 reacted to the apical cell membrane of superficial cells and the intercellular space of superficial and wing cells. No immune reactivity of mAb KL-6 was observed in the basal plasma membrane of basal epithelial cells. Results of impression cytology indicated that the corneal epithelium of 13 of 24 non-dry eyes was weakly stained by mAb KL-6, whereas 42 of 43 dry eyes showed a mosaic pattern. In non-dry eyes, 19 of 24 bulbar conjunctival epithelia expressed the KL-6 epitope in a honeycomb pattern. In mild (17/19) and moderate (17/17) dry eye conjunctiva, the KL-6 epitope showed a mosaic pattern. However, the expression of KL-6 epitope decreased in severe dry eyes, showing a mo...Continue Reading
Citations
Sep 1, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Nobuyoshi KitaichiShigeaki Ohno
May 18, 2007·The Ocular Surface
Nov 18, 2011·Cornea·Ilene K GipsonDebra Schaumberg
Aug 5, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Padmapriya Ramamoorthy, Jason J Nichols
Jun 28, 2011·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Lele CuiFan Lu
Jan 6, 2011·Development·Danielle MatsushimaLarysa H Pevny
May 15, 2012·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Hiroko Uchida, Yoshichika Imanaga
Jan 29, 2010·The Ocular Surface·Ana Guzman-Aranguez, Pablo Argüeso
Jul 6, 2011·Experimental Eye Research·Pooja JainKaren Meerovitch
Dec 13, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Satoru KaseShigeaki Ohno
Jan 10, 2014·Current Eye Research·Kinya IwatsukaKazuaki Kakehi
Dec 2, 2004·Developmental Biology·Yasuhito HayashiWinston Whei-Yang Kao
May 8, 2016·The Ocular Surface·Anna F Ablamowicz, Jason J Nichols
Apr 6, 2017·PloS One·Lukas L NegrinThomas Heinz
Nov 28, 2018·Investigative Ophthalmology & Visual Science·Yuichi Uchino
Aug 27, 2013·Current Opinion in Allergy and Clinical Immunology·Flavio MantelliPablo Argüeso
Aug 31, 2019·Eye & Contact Lens·Pablo Argüeso
Aug 13, 2015·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Denise N Stephens, Nancy A McNamara
Sep 12, 2019·PloS One·Arisa MitaniYuichi Ohashi
Mar 30, 2021·The Ocular Surface·Rafael Martinez-CarrascoM Elizabeth Fini
Jul 22, 2019·Experimental Eye Research·Céline PortalJean-Luc Desseyn