Expression profile and specific network features of the apoptotic machinery explain relapse of acute myeloid leukemia after chemotherapy.

BMC Cancer
Marco RagusaMichele Purrello

Abstract

According to the different sensitivity of their bone marrow CD34+ cells to in vitro treatment with Etoposide or Mafosfamide, Acute Myeloid Leukaemia (AML) patients in apparent complete remission (CR) after chemotherapy induction may be classified into three groups: (i) normally responsive; (ii) chemoresistant; (iii) highly chemosensitive. This inversely correlates with in vivo CD34+ mobilization and, interestingly, also with the prognosis of the disease: patients showing a good mobilizing activity are resistant to chemotherapy and subject to significantly higher rates of Minimal Residual Disease (MRD) and relapse than the others. Based on its known role in patients' response to chemotherapy, we hypothesized an involvement of the Apoptotic Machinery (AM) in these phenotypic features. To investigate the molecular bases of the differential chemosensitivity of bone marrow hematopoietic stem cells (HSC) in CR AML patients, and the relationship between chemosensitivity, mobilizing activity and relapse rates, we analyzed their AM expression profile by performing Real Time RT-PCR of 84 AM genes in CD34+ pools from the two extreme classes of patients (i.e., chemoresistant and highly chemosensitive), and compared them with normal control...Continue Reading

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Citations

Mar 27, 2012·Leukemia·E LionE L J Smits
Mar 17, 2012·Nature Protocols·Nadezhda T DonchevaMario Albrecht
Dec 22, 2012·Immunotherapy·Felix S LichteneggerMarion Subklewe
Mar 29, 2014·Frontiers in Immunology·Salem ChouaibJan Spanholtz
Sep 11, 2020·Cell Biology International·Yasin BagheriMehdi Yousefi

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Methods Mentioned

BETA
Assay
light microscopy
PCR

Software Mentioned

APID2NET
Network Analyzer
MultiExperiment Viewer
cytoscape

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