Expression profile of Gli family members and Shh in normal and mutant mouse limb development

Developmental Dynamics : an Official Publication of the American Association of Anatomists
D Büscher, U Rüther

Abstract

Gli genes represent a small family, encoding zinc-finger proteins of the Krüppel-type. The family consists of Gli(1), Gli2, and Gli3, all of which are expressed in the developing mouse limb bud. To assess the role of the Gli family and Sonic hedgehog (Shh) in mouse limb development, we compared the expression domains of all three Gli genes and of Shh. Although each Gli gene has its own distinct expression pattern in limb buds, at 10.5-11.5 dpc all three genes were found not to be expressed in the posterior region, the presumptive Shh expression domain. This transient mutually exclusive expression suggested a potential interaction between Gli genes and Shh. To address this matter, we analysed the expression of Gli genes and Shh in two polydactyly mouse mutants, Extra toes (Xt) and Hemimelic-extra toes (Hx) which express Shh ectopically in the anterior region of the limb field. Since Xt mice lack Gli3 expression, the ectopic Shh expression is genetically linked to the absence of Gli3. In Hx mice we found a down-regulation of Gli3 in the anterior region of the limb bud. In both mutants Gli2 expression pattern was not altered, whereas Gli1 expression was anteriorly up-regulated adjacent to the ectopic Shh domain. These results stro...Continue Reading

References

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A VortkampK H Grzeschik
Feb 1, 1990·Molecular and Cellular Biology·K W Kinzler, B Vogelstein
Oct 1, 1990·Molecular and Cellular Biology·J M RuppertK W Kinzler
Jun 1, 1990·Genes & Development·S Eaton, T B Kornberg
Aug 1, 1988·Molecular and Cellular Biology·J M RuppertB Vogelstein
Jul 1, 1995·Genes & Development·H MasuyaT Shiroishi
Jul 1, 1993·Mechanisms of Development·A M TaylorP W Ingham
Mar 18, 1993·Nature·E R AngertN R Pace
Jul 17, 1996·Biochimica Et Biophysica Acta·H ThienU Rüther
Nov 14, 1996·Nature·V MarigoC J Tabin
Mar 1, 1997·Mechanisms of Development·D BüscherU Rüther

❮ Previous
Next ❯

Citations

May 16, 2008·Experimental Biology and Medicine·Brad E Morrison, Santosh R D'Mello
Aug 11, 1999·Human Molecular Genetics·M Kalff-SuskeK H Grzeschik
Jun 14, 2015·Human Molecular Genetics·Christine LaclefSylvie Schneider-Maunoury
Oct 27, 1999·Mechanisms of Development·J Heymer, U Rüther
Mar 16, 2017·Frontiers in Cell and Developmental Biology·Cheryll Tickle, Matthew Towers
Jan 14, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M IwamotoK Kurisu
Jan 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·X CaubitS Krauss
May 22, 2001·Clinical Genetics·J KimC C Hui
Jul 21, 2004·American Journal of Medical Genetics. Part a·Anne V HingMichael L Cunningham
Apr 12, 2002·Genesis : the Journal of Genetics and Development·Elisabeth H VillavicencioPhilip M Iannaccone
Apr 18, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Qihong ZhangBradley K Yoder
May 28, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Sukanya LodhNorann A Zaghloul
Mar 3, 2005·Anatomy and Embryology·Christoph HornikErnst-Martin Füchtbauer
Aug 31, 2018·International Journal of Molecular Sciences·Maja SabolSonja Levanat
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sonja K NielsenSøren Tvorup Christensen
Mar 6, 2010·Congenital Anomalies·Ichiro NaruseSatoshi Ishikiriyama
Nov 26, 2009·American Journal of Medical Genetics. Part a·Ariana KariminejadMohamad Hasan Kariminejad
Jun 15, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Thomas B Knudsen, Devendra M Kochhar
Dec 26, 2012·The Journal of Biological Chemistry·Anastassia VoronovaIlona S Skerjanc
Dec 13, 2012·Development, Growth & Differentiation·Takayuki Suzuki
Feb 1, 2005·Clinical Genetics·A TalamilloM A Ros
Apr 15, 2011·Development·Laurianne BesseSylvie Schneider-Maunoury
Nov 27, 1999·Current Biology : CB·C von Mering, K Basler
Dec 19, 2000·Mechanisms of Development·P KrausC A Loomis
Sep 11, 2002·Developmental Biology·Marilyn L LammWade Bushman
Jan 12, 1999·Neuron·L V Goodrich, M P Scott
Jul 29, 2021·Cellular Oncology (Dordrecht)·Chun YangPierre Hardy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.