Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus)

Frontiers in Microbiology
Irene K MekiAdly M M Abd-Alla

Abstract

The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly's fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evide...Continue Reading

References

May 29, 2003·Molecular Cell·Yonatan GradJohn Kim
Oct 9, 1959·Science·E F KNIPLING
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chulan KwonDeepak Srivastava
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Nov 10, 2007·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Jul 8, 2008·PLoS Pathogens·Zhiyong XiGeorge Dimopoulos
Jul 11, 2008·Journal of Virology·Mazhar HussainSassan Asgari
Jun 10, 2009·Insect Biochemistry and Molecular Biology·Guy CaljonJan Van Den Abbeele
Jul 22, 2009·Developmental Cell·Nicola IovinoUlrike Gaul
Oct 23, 2009·Journal of Virology·Mazhar Hussain, Sassan Asgari
May 19, 2010·Annual Review of Microbiology·Rebecca L Skalsky, Bryan R Cullen
Feb 22, 2011·Virology·Aron R MarquitzNancy Raab-Traub
Mar 25, 2011·Methods in Molecular Biology·Adam Grundhoff
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mazhar HussainSassan Asgari
Sep 3, 2011·Frontiers in Physiology·Sassan Asgari
Sep 14, 2011·PLoS Neglected Tropical Diseases·Adly M M Abd-AllaMax Bergoin
Nov 26, 2011·Algorithms for Molecular Biology : AMB·Ronny LorenzIvo L Hofacker
Jun 23, 2012·PloS One·Geng YangXiaobo Zhang
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alfred W BronkhorstRonald P van Rij
Nov 21, 2012·Insect Biochemistry and Molecular Biology·Keira Lucas, Alexander S Raikhel
Jan 12, 2013·PLoS Pathogens·Rodney P Kincaid, Christopher S Sullivan
Feb 15, 2013·The Journal of General Virology·Mohammad MehrabadiSassan Asgari
May 23, 2013·Journal of Cellular Physiology·Julian C RobertsKehinde Ross
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guangmei ZhangSassan Asgari
Oct 15, 2013·Journal of Molecular Biology·Megan B KingsolverRichard W Hardy
Nov 8, 2013·Journal of Virology·Yaodong HeXiaobo Zhang
Sep 25, 2014·Viruses·Sassan Asgari
Jan 23, 2015·PLoS Neglected Tropical Diseases·Payal D MaharajSaravanan Thangamani

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP139935
EF568108
GPAI025158

Methods Mentioned

BETA
dissections
PCR
Illumina sequencing
GTPase

Software Mentioned

BLASTp
RNA22
VMir
IIID
ggplot2
CLC genomic workbench
lattice
RNAfold
MASS
Blast2GO

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.