Expression profile of matricellular proteins in hypertrophied right ventricle of monocrotaline-induced pulmonary hypertensive rats

The Journal of Veterinary Medical Science
Keisuke ImotoHideyuki Yamawaki

Abstract

Matricellular proteins, a non-structural extracellular matrix (ECM) component, bind to and modulate various molecules including growth factor, cytokine, protease, other ECM components and cell membrane receptors. While most matricellular proteins are hardly expressed in normal adult tissue, they are re-expressed in heart tissue during cardiac diseases. The present study aimed to clarify the mRNA expression profile of matricellular proteins [secreted protein acidic and rich in cysteine: SPARC, hevin, thrombospondin (TSP)-1, -2 and -4, CCN1 and 5, tenascin (Tn) C and N, periostin and osteopontin (OPN)] in hypertrophied right ventricle (RV) of monocrotaline (MCT)-induced pulmonary hypertensive rats. Male Wistar rats were intraperitoneally treated with MCT or saline. Two or three weeks after MCT treatment, echocardiography was performed, and mRNA expression of matricellular proteins was measured by real-time polymerase chain reaction. MCT (2 weeks) induced pulmonary hypertension, RV dysfunction and hypertrophy, which were all worsened 3 weeks after MCT treatment. Expression of mRNA for SPARC, hevin, TnC, TSP-1, -2 and -4, CCN1 and 5, periostin and OPN but not TnN was significantly upregulated in RV of MCT (2 weeks)-treated rats. Ex...Continue Reading

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Citations

Jul 18, 2017·International Journal of Molecular Sciences·Dimitry A ChistiakovAlexander N Orekhov
Jun 4, 2018·Pflügers Archiv : European journal of physiology·Keisuke ImotoHideyuki Yamawaki
Apr 7, 2020·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Stanislav KeranovHolger Nef
Sep 25, 2018·Neurological Research·Hiroko Ikeshima-KataokaToshimitsu Uede
Oct 9, 2020·Circulation Research·Peiran Yang, Paul B Yu
Jun 16, 2021·American Journal of Respiratory Cell and Molecular Biology·Anjira S AmbadeRachel L Damico

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Methods Mentioned

BETA
PCR
gene knockdown

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