Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for Phytophthora resistance

Proceedings. Biological Sciences
Sophie de VriesLaura E Rose

Abstract

Plants possess a battery of specific pathogen resistance (R-)genes. Precise R-gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of R-genes, nucleotide-binding site leucine-rich repeats (NBS-LRRs). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of R-genes. Despite this transcriptional release of R-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens. To investigate this paradox, we analysed how the regulation of NBS-LRRs by miR482/2118 has been shaped by the coevolution between Phytophthora infestans and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their NBS-LRR targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of NBS-LRRs in Solanum lycopersicum. Based on miR482/2118 expression profiling in two additional tomato species-with different coevolutionary histories with P. infestans-we hypothesize that pathogen-mediated RNA silencing suppression is most effective in th...Continue Reading

References

Jan 19, 2002·Genes & Development·Trevor L StokesEric J Richards
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Junqi SongJiming Jiang
Dec 14, 2004·Science·Rebecca L AllenJim L Beynon
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Thirumala-Devi KannegantiSophien Kamoun
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Yongqing LiJian Hua
Apr 22, 2008·Nucleic Acids Research·Andreas R GruberIvo L Hofacker
Aug 30, 2008·Evolution; International Journal of Organic Evolution·Leonie C Moyle
Apr 1, 1997·Phytopathology·G A ForbesW E Fry
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Brendan S KelleyJocelyn K C Rose
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Feng LiBarbara Baker
Mar 13, 2012·The Plant Cell·Padubidri V ShivaprasadDavid C Baulcombe
Feb 5, 2013·Nature Genetics·Yongli QiaoWenbo Ma
Aug 8, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chunzhi ZhangJunming Li
Apr 24, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chunzhi ZhangYongchen Du
Oct 21, 2014·PLoS Pathogens·Shouqiang OuyangKatherine A Borkovich
Feb 11, 2015·Current Opinion in Immunology·Xin LiYuelin Zhang
Aug 12, 2016·Molecular Biology and Evolution·Yu ZhangBlake C Meyers
Nov 1, 2016·Genome Biology and Evolution·Ming WenTian Tang
Jan 13, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bruce A McDonald, Eva H Stukenbrock
Aug 1, 2007·Plant Disease·Christine D SmartWilliam E Fry

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Citations

Dec 24, 2018·The New Phytologist·Guan-Zhu Han
Aug 10, 2019·Molecular Biology and Evolution·Paolo FranchiniAxel Meyer
May 21, 2020·The Plant Journal : for Cell and Molecular Biology·Ning JiangYushi Luan
Sep 15, 2018·Communicative & Integrative Biology·Sophie de VriesClaudio H Slamovits
Mar 19, 2019·European Journal of Plant Pathology·Laura E RoseMireille van Damme
Sep 11, 2020·Molecular Biology Reports·Charu KulshresthaJebi Sudan
Jan 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Alex Canto-PastorDavid C Baulcombe
Apr 4, 2021·International Journal of Molecular Sciences·Xiaoqian YangYanwei Wang
Oct 5, 2021·The New Phytologist·Xinyi HuChristina Dixelius

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Methods Mentioned

BETA
PCR

Software Mentioned

RNAfold
BLASTn
R
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