Expression profiling and functional characterization of the duplicated Oxr1b gene in zebrafish.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Hao XuYun Li

Abstract

Oxidation Resistance Gene 1 (OXR1) is a conserved gene family involved in protecting various species against oxidative stress. The zebrafish expresses a pair of OXR1 paralogs (i.e., oxr1a and oxr1b). Our previous work has revealed the importance of oxr1a in regulating antioxidant defenses during oxidative stress, but the role of oxr1b is remains unknown. Herein we reported the spatial-temporal expression of oxr1b and revealed its function through reverse genetics. The results showed that, as with oxr1a, oxr1b is a typical maternal-zygotic gene. Its mRNA is mainly distributed in the eye, brain and nervous system (e.g., anterior/posterior lateral line ganglion, neuromasts and spinal cord neuron). Although oxr1a and oxr1b genes have similar expression patterns during embryonic development, the latter have higher levels at the corresponding stages. Subsequently, a viable oxr1b-/- mutant was generated by the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9) system. Oxr1b knockout caused multiple antioxidant genes (i.e., gpx4a, gpx4b, sod1 and sod3b) to be downregulated, resulting in hypersensitive to oxidative stress. Furthermore, by comparative transcriptome analysis, we found that ...Continue Reading

References

Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M R VolkertD E Housman
Sep 5, 2001·Science·M Long, K Thornton
Nov 25, 2003·Nature Reviews. Genetics·Manyuan LongWen Wang
Apr 3, 2004·Molecular and Cellular Biology·Nathan A Elliott, Michael R Volkert
Apr 27, 2004·Nature Genetics·Sarah A Teichmann, M Madan Babu
May 25, 2004·The Journal of Biological Chemistry·Stéphane DesaintMichel B Toledano
Sep 15, 2004·Trends in Genetics : TIG·John PostlethwaitYi-Lin Yan
Dec 18, 2004·Methods in Cell Biology·John PostlethwaitPeter J Tonellato
Aug 18, 2005·Journal of Cell Science·Maosong Qi, Elaine A Elion
Jul 18, 2009·Current Opinion in Genetics & Development·Elliott W Abrams, Mary C Mullins
Sep 6, 2012·Molecular and Cellular Biology·Katsuki MukaigasaMakoto Kobayashi
Apr 10, 2013·Gene Expression Patterns : GEP·F J F LarocheH P Spaink
Apr 12, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoshi OtaAtsuo Kawahara
Mar 11, 2015·Brain : a Journal of Neurology·Kevin X LiuPeter L Oliver
Sep 12, 2015·Current Topics in Developmental Biology·Valeria Yartseva, Antonio J Giraldez

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